| 合川戴氏霉菌株产纤维素酶活力的条件优化 |
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| 引用本文： | 韩燕峰,王宝林,陈万浩,王玉荣,杜文,梁宗琦.合川戴氏霉菌株产纤维素酶活力的条件优化[J].贵州农业科学,2012,40(7):109-111. |
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| 作者姓名： | 韩燕峰 王宝林 陈万浩 王玉荣 杜文 梁宗琦 |
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| 作者单位： | 贵州大学生命科学学院真菌资源研究所,贵州贵阳,550025 |
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| 基金项目： | 国家自然科学基金项目“中国西部地区金孢属资源物种多样性及其应用价值研究”，贵州省教育厅自然科学基金项目“土壤微生物产维生素的调查研究” |
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| 摘 要： | 为耐高温真菌资源的开发利用提供参考依据,在查氏基础培养基上,进行了提高合川戴氏霉GZUIFR-H08-1菌株产纤维素酶活力的条件优化试验。结果表明:合川戴氏霉GZUIFR-H08-1产纤维素酶的最佳碳源为可溶性淀粉20g/L,氮源为氯化铵2.8g/L,初始pH 8.0,金属离子为Ca2+5mmol/L。在此发酵条件下,其酶活力达558.73U,比初筛时的酶活力高2.44倍。
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| 关 键 词： | 戴氏霉 耐高温 纤维素酶 优化条件 |
Condition Optimization of Cellulase-producing Activity of Taifanglania hechuanensis |
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| HAN Yan-feng
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WANG Bao-lin
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CHEN Wan-hao
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WANG Yu-rong
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DU Wen
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LIANG Zong-qi.Condition Optimization of Cellulase-producing Activity of Taifanglania hechuanensis[J].Guizhou Agricultural Sciences,2012,40(7):109-111. |
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| Authors: | HAN Yan-feng WANG Bao-lin CHEN Wan-hao WANG Yu-rong DU Wen LIANG Zong-qi |
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| Institution: | (Institute of Fungus Resources,College of Life Science,Guizhou University,Guiyang,Guizhou 550025,China) |
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| Abstract: | A condition optimization test for improving the cellulase-producing activity of T.hechuanensis GZUIFR-H08-1 was conducted based on the Czapek basal medium to provide a reference for the development and utilization of the thermotolerant fungi resources.The results showed that its cellulase activity was up to 558.73 U,which was 2.44 times as that of preliminary screening in the following conditions: soluble starch 20 g/L,NH4Cl 2.8 g/L,initial pH 8.0,Ca2+ 5 mmol/L. |
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| Keywords: | Taifanglania thermotolerant cellulase orthogonal design |
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