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两个西南马种GHR基因第10外显子SSCP分析
引用本文:张涛,路宏朝,薛贤裔.两个西南马种GHR基因第10外显子SSCP分析[J].广东农业科学,2010,37(2):149-152.
作者姓名:张涛  路宏朝  薛贤裔
作者单位:陕西理工学院生物科学与工程学院,陕西,汉中,723000
基金项目:陕西省教育厅科研项目(07JK212);;陕西理工学院科研项目(SLGQD0513)
摘    要:通过PCR-SSCP检测10匹宁强矮马和12匹贵州马GHR基因第10外显子的多态性分析,结果表明,宁强矮马和贵州马GHR基因外显子10的第2和第4区域的保守性较高,基因多样性相对较低,第1和第3区的遗传变异较大,基因多样性水平较高。分析比较发现宁强矮马GHR基因外显子10的多态性比贵州马稍高,首次表明宁强矮马在GHR10上存在较大的遗传多态性。

关 键 词:宁强矮马  贵州马  GHR基因第10外显子  PCR-SSCP  

Single-strand Conformation Polymorphism Analysis of tenth exon of growth hormone receptor gene in two southwest horse breeds
ZHANG Tao,LU Hong-zhao,XUE Xian-yi.Single-strand Conformation Polymorphism Analysis of tenth exon of growth hormone receptor gene in two southwest horse breeds[J].Guangdong Agricultural Sciences,2010,37(2):149-152.
Authors:ZHANG Tao  LU Hong-zhao  XUE Xian-yi
Institution:School of Biological Science and Engineering;Shanxi University of Technology;Hanzhong 723000;China
Abstract:The polymorphism of horse growth hormone receptor gene in exon10 in 22 horsess including Ningqiang pony and Guizhou horse were detected by PCR-SSCP with four different pairs of primers.The results showed that the conservatism of the second and fourth region of GHR10 was higher in Ningqiang pony and Guizhou horse,the level of diversity of GHR10 was relatively lower.The genetic variation of the second and fourth region of GHR10 was comparatively larger;the level of diversity of GHR10 was relatively higher.The...
Keywords:Ningqiang pony  Guizhou horse  the tenth exon of growth hormone receptor gene  PCR-SSCP  
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