| 四环素全抗原的合成及多克隆抗体的制备 |
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| 引用本文： | 包晓玮,韩海霞,景伟文,杨晓君,朱金芳,陆建胜.四环素全抗原的合成及多克隆抗体的制备[J].广东农业科学,2010,37(5):171-173. |
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| 作者姓名： | 包晓玮 韩海霞 景伟文 杨晓君 朱金芳 陆建胜 |
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| 作者单位： | 新疆农业大学药学院,新疆,乌鲁木齐,830052 |
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| 基金项目： | 克拉玛依市农牧局资助项目 |
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| 摘 要： | 利用Mannich反应合成四环素(Tc)与BSA(牛血清白蛋白)的完全抗原,由紫外光谱确定是否偶联成功;同理,制备四环素OVA抗原作为包被抗原,以制备的完全抗原TC-BSA免疫清洁级新西兰兔制备多克隆抗体,运用间接ELISA法测定血清抗体效价。试验结果表明,紫外光谱确定偶联成功,血清抗体效价为105,间接竞争法测定抗体50%抑制率(IC50)检测限为200ng/mL,最低检测限量可达到20ng/mL,特异性好。
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| 关 键 词： | 四环素 全抗原 多克隆抗体 |
Synthesis of antigens of tetracycline and preparation of polyclonal antibodies |
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| BAO Xiao-wei,HAN Hai-xia,JING Wei-wen,YANG Xiao-jun,ZHU Jin-fang,LU Jian-Sheng.Synthesis of antigens of tetracycline and preparation of polyclonal antibodies[J].Guangdong Agricultural Sciences,2010,37(5):171-173. |
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| Authors: | BAO Xiao-wei HAN Hai-xia JING Wei-wen YANG Xiao-jun ZHU Jin-fang LU Jian-Sheng |
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| Institution: | BAO Xiao-wei,HAN Hai-xia,JING Wei-wen,YANG Xiao-jun,ZHU Jin-fang,LU Jian-Sheng (College of Pharmaceutical Science of Xinjiang Agriculture University,Urumqi 830052,China) |
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| Abstract: | Tetracycline-BSA immunoantigens( TC-BSA)were prepared by Mannichmethod,the successful linkage of the TC-BSA was identified by UV.Similarly, Tetracycline-OVA packantigens were prepared .TC-BSA was injected into rabbit as immunogen four time in order to prepare different polyclonal antibodies.The special antibody of Tetracycline ( TC)was identified by indirect enzyme-linked immunosorbent assay ( ELISA). The results showed that the working concentration of the two kinds of the special antibodieswas 105, the Te... |
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| Keywords: | ELISA |
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