| 马铃薯X病毒贵州分离物的分子鉴定 |
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| 引用本文： | 姚东校,洪鲲,杨立昌,乙引,万晴姣.马铃薯X病毒贵州分离物的分子鉴定[J].广东农业科学,2013,40(13):139-141. |
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| 作者姓名： | 姚东校 洪鲲 杨立昌 乙引 万晴姣 |
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| 作者单位： | 1. 贵州师范大学生命科学学院,贵州贵阳,550001
2. 贵州师范大学生命科学学院,贵州贵阳550001;贵州省植物生理与发育调控重点实验室,贵州贵阳550001 |
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| 基金项目： | 贵州省重点实验室项目，长江学者和创新团队发展计划项目，贵阳市星火计划项目，贵州省社发科技攻关项目，贵州省农业科技攻关项目，贵州省科技联合基金 |
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| 摘 要： | 从贵州威宁县染病马铃薯植株中分离得到马铃薯X病毒贵州分离物(PVX-GZ),采用RT-PCR扩增并克隆了该分离物的外壳蛋白基因(CP).经测序,该CP基因长714 bp,编码237个氨基酸残基,分子量25.2 ku;与19种已报道的PVX各地分离物的同源性相比,其核苷酸和氨基酸序列分别在78.6％～97.5％和84.8％～99.6％之间,其中,与两种典型的PVX X3株系(荷兰分离物X3和英国分离物UK3)的基因序列同源性达97.1％以上.结合寄主鉴别结果,判断该分离物属PVX的X3株系.
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| 关 键 词： | 马铃薯X病毒 外壳蛋白 亲缘分析 |
Molecular identification of a potato virus X isolated from potatos in Guizhou |
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| YAO Dong-xiao
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HONG Kun
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YANG Li-chang
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YI Yin
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WAN Qing-jiao.Molecular identification of a potato virus X isolated from potatos in Guizhou[J].Guangdong Agricultural Sciences,2013,40(13):139-141. |
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| Authors: | YAO Dong-xiao HONG Kun YANG Li-chang YI Yin WAN Qing-jiao |
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| Abstract: | The coat protein (CP) gene of a potato virus X (PVX-GZ) isolated from potato in Guizhou was cloned and sequenced. The CP gene was 714 bp long and encoded a 237 amino acid residues with a predicted molecular mass of 25.2 ku. Sequence comparison results showed that the nucleotide and deduced amino acids sequences of the gene share homology with 19 reported PVX isolates were 78.6%~97.5% and 84.8%~99.6%, respectively. The highest nucleotide sequence homology (over 97.1%) was reached when compared with the isolated X3 from Holland and UK3 from UK, two representative isolates of PVX strain X3. Consistent results from differential host test revealed that PVX-GZ belonged to strain X3. |
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| Keywords: | potato virus X coat protein phylogenetic analysis |
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