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西兰花花茎的组织培养及快繁技术研究
引用本文:韩德伟,王振龙,关丽霞,修红旭.西兰花花茎的组织培养及快繁技术研究[J].广东农业科学,2010,37(2):46-48.
作者姓名:韩德伟  王振龙  关丽霞  修红旭
作者单位:辽宁农业职业技术学院生物技术系,辽宁,营口,115009
摘    要:以西兰花花茎为外植体,进行不定芽诱导、增殖及植株再生的研究。结果表明,花茎切割后平接或按极性竖接种于培养基中两种接种方式对不定芽的诱导无明显差异,不定芽最佳诱导培养基为MS+6-BA2.0mg/L+NAA0.3mg/L,最适增殖培养基为MS+6-BA1.0mg/L+NAA0.1mg/L,最适生根培养基是1/2MS+IBA0.1mg/L+NAA0.05mg/L+活性炭0.5g/L。

关 键 词:西兰花  花茎  组织培养  

Study on tissue culture and rapid propagation of Brassica oleracea var. italica Pianch scape
HAN De-wei,WANG Zhen-long,GUAN Li-xia,XIU Hong-xu.Study on tissue culture and rapid propagation of Brassica oleracea var. italica Pianch scape[J].Guangdong Agricultural Sciences,2010,37(2):46-48.
Authors:HAN De-wei  WANG Zhen-long  GUAN Li-xia  XIU Hong-xu
Institution:Biotechnology Department;Liaoning Agricultural Vocation-Technical College;Yingkou 115009;China
Abstract:The scape of Brassica oleracea var.italica Pianch was used as explants for adventitious buds induction,multiplication and plant regeneration.The results showed that there was no significant difference between the two inoculation methods on induction of adventitious buds after the cross-cutting plants inoculated or inoculated in the medium polarity erection.The optimum medium for adventitious buds induction was MS+6-BA2.0 mg/L+NAA0.3 mg/L.The appropriate medium for multiplication and plant regeneration were ...
Keywords:Brassica oleracea var  italica Pianch  scape  tissue culture  
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