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植物二酰甘油酰基转移酶及其编码基因研究进展
引用本文:王婷婷,马晓林,李凤兰,郭惠红,陈发菊.植物二酰甘油酰基转移酶及其编码基因研究进展[J].广东农业科学,2012,39(6):127-130.
作者姓名:王婷婷  马晓林  李凤兰  郭惠红  陈发菊
作者单位:1. 北京林业大学生物科学与技术学院/林木育种国家工程实验室/林木花卉遗传育种教育部重点实验室/国家林业局林木花卉育种与生物工程重点开放实验室,北京,100083
2. 北京市海淀区圆明园管理处,北京,100083
3. 三峡大学生物技术研究中心,湖北宜昌,443002
基金项目:北京林业大学林木育种国家工程实验室开放课题,湖北省自然科学基金重点项目子课题
摘    要:三酰甘油(TAG)是植物的主要储藏脂类,二酰甘油酰基转移酶(DGATs)催化TAG生物合成的最后一步乙酰化反应,使二酰甘油加上一个脂肪酰基形成TAG。因此,DGATs被认为是TAG生物合成过程中最关键的限速酶。重点介绍了植物DGATs及其编码基因的分类、结构以及在植物油脂合成中的作用及应用的最新研究进展,以期为应用基因工程等技术提高植物油脂(如种子油)含量或改善油脂品质提供参考。

关 键 词:二酰甘油酰基转移酶  编码基因  研究进展

Advances in plant diacylglycerol acyltransferases and the coding genes
WANG Ting-ting , MA Xiao-lin , LI Feng-lan , GUO Hui-hong , CHEN Fa-ju.Advances in plant diacylglycerol acyltransferases and the coding genes[J].Guangdong Agricultural Sciences,2012,39(6):127-130.
Authors:WANG Ting-ting  MA Xiao-lin  LI Feng-lan  GUO Hui-hong  CHEN Fa-ju
Institution:1.College of Biological Sciences and Biotechnology,Beijing Forestry University/National Engineering Laboratory for Tree Breeding/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education/The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration,Beijing 100083,China;2.Yuanmingyuan Administration,Beijing Haidian District,beijing 100083,China;3.Biotechnology Research Center,Three Gorges University,Yichang 443002,China)
Abstract:Triacylglycerol(TAG) is the main storage lipid in plants.Diacylglycerol acyltransferases(DGATs) catalyze the final step of TAG biosynthesis by transferring an acyl group from fatty acyl-CoA to the diacylglycerol backbone.Thus,DGATs are thought to be the most critical rate-limiting enzymes in TAG biosynthesis.In this paper,the latest research advances in the classifications and structures of DGATs and the coding genes as well as their functions and applications in TAG biosynthesis were reviewed,which could be helpful for improving the quality and quantity of plant storage lipids such as seed oils by genetic manipulations.
Keywords:diacylglycerol acyltransferases(DGATs)  coding genes  advances
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