基于线粒体COⅡ基因的3 种土白蚁系统发育分析

对海南的3 种土白蚁15 个不同地理种群的线粒体CO域基因进行了克隆鉴定和序列分析，并结合 GenBank 中的数据，构建进化树进行遗传变异分析。结果表明，3 种土白蚁种间同源性为92.5%~95.1%，遗传分歧为 5.1%~8.1%；黑翅土白蚁和海南土白蚁CO域基因不同种群之间的同源性分别为97%~99.9%和98.4%~100%，遗传分 歧分别为0.1%~3.0%和0%~1.6%。崖城的一个未知种群Odontotermes sp. 与来自日本的O. javanicus 同源性最高 (95.4%）。土白蚁CO域基因序列中AT 含量为61.4%，高于CG 含量，碱基变异的主要形式为颠换。

Phylogenetic analysis of three Odontotermes holmgern based on mitochondrial COn sequences

Phylogenetic and sequence analysis of Cytochrome Oxidase Subunit II gene (CO gene) were conducted to elucidate the relationships among 15 populations of 3 species Odontotermes from Hainan province. The results showed that the sequence identification of inter-species of Odontotermes ranged from 92.5% to 95.1%, and divergence ranged from 5.1% to 8.1%; while different populations of O. formosanus and O. hainanensis ranged from 97% to 99.9% and 98.4% to 100%, and divergence ranged from 0.1% to 3.0% and 0% to 1.6%. The sequence of Odontotermes sp. Yach shared 95.4% identification and closely genetic relationship with O. javanicus, a species from Japan. CO gene of genus Odontotermes showed high AT composition, which reached to 61.4%, and the main base variation was transversion .