宁薯10号茎尖离体培养及种薯生产

为了保持宁薯10号甘薯品种的优良种性，延长种薯的田间应用年限，运用茎尖分生组织培养，指示植物检测方法获得脱毒苗。结果表明，以MS+6-BA 1.0 mg/L+NAA0.1 mg/L为宁薯10号茎尖初代培养基，成苗率达94.4%；以6-BA0.2 mg/L+NAA0.05 mg/L为茎切段继代增殖培养基，繁殖系数达2.54倍；宁薯10号脱毒苗产量比对照原品种提高16.5%，达到极显著水平，可大面积推广应用。

In vitro micropropagatiing of apical meristem and seed breeding to the 10th Ningde-sweet potato

To keep the good character of 10th Ningde-sweet potato, extend the seed application experience in field, virus-free plantlets was obtained by apical meristem culture and indicator plant detection method. The experimental results showed that the first culture medium for apical meristem growth of the 10th Ningde-sweet potato was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L, seedling rate up to 94.4%, and subculture medium for stem segments propagating was 6-BA 0.2 mg/L+NAA 0.05 mg/L, the propagation coefficient was up to 5 times. The yield of its virus-free seedling increased by 16.5% than that of the control original varieties, reached extremely significant level, so it could be popularized in large area.