藏猪耳部成纤维细胞的分离与培养

用组织块法分离培养藏猪耳部成纤维细胞，并进行体外传代和冷冻，冷冻复苏后的藏猪耳部成纤维细胞仍可以正常培养和传代，冻存前后活率都在90%以上；其生长曲线呈典型的“S”型，细胞接种后，在经历0-2 d的潜伏期后，第3 d细胞开始迅速生长，进入对数生长期，到第10 d细胞开始发生接触抑制，生长缓慢进入平台期。研究结果表明，已成功分离培养到藏猪的耳部成纤维细胞，这将为藏猪的体细胞克隆和保种等方面的研究奠定基础。

Dissociation and culture of ear marginal fibroblasts in Tibetan pigs

The study was to isolate and culture fibroblast cell lines of ear marginal tissues in Tibetan pigs by direct adherent culture methods in vitro. The results showed that fibroblasts were successfully isolated and could be used for passage and freezing; the cells could still be cultured and subcultured after freezing-thawing, both of the survival rates of cells before and after freezing were above 90%; the growth curve was typical of S type, the incubation period was 0-2 d, the logarithmic growth phase was 3-9 d, and the plateau was 10-12 d after the cells inoculation. The ear fibroblasts in Tibetan pigs were successfully cultured. The result of this study could lay the foundation for research on somatic cell cloning and breed conservation of Tibetan pigs.