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鸟粪石沉淀法改善微藻氨絮凝的后续培养
引用本文:刘晨风,刘智永,侯余勇,杨振华,温泽文,肖冬光,张娟琨,陈方见.鸟粪石沉淀法改善微藻氨絮凝的后续培养[J].广东农业科学,2013,40(10):186-190.
作者姓名:刘晨风  刘智永  侯余勇  杨振华  温泽文  肖冬光  张娟琨  陈方见
作者单位:天津科技大学生物工程学院;中国科学院天津工业生物技术研究所天津市工业生物系统与过程工程重点实验室
基金项目:国家973计划项目(2011CB200905);国家自然科学基金(31200093)
摘    要:为高效回收氨絮凝后的氨氮并改善其后续培养,研究了鸟粪石沉淀法回收氨氮问题并比较了后续培养中不同营养组分补加对微藻生长的影响。结果表明,鸟粪石沉淀法可解决微藻氨絮凝的氨氮回收问题,回收率达98.8%;N1和N5组的营养添加组合最适合Nannochlropsis oculata的后续培养,N1组微藻的生物量产率、蛋白质含量、类胡萝卜素含量上分别是对照组的1.0、2.1、6.0倍,N5组微藻的生物量产率、蛋白质含量、类胡萝卜素含量上分别是对照组的1.4、1.6、4.7倍。后续培养的藻液仍可利用氨絮凝法沉降采收,其絮凝率在10min内达到90%以上。因此,鸟粪石沉淀法能有效地改善微藻氨絮凝的后续培养。

关 键 词:微藻  鸟粪石沉淀法  氨絮凝  后续培养

Struvite precitation improving subsequent culture of microalgae with ammonia-flocculation treatment
Abstract:Struvite precipitation was used to study the highly efficient recovery of ammonia-nitrogen from the ammonia-flocculated medium of microalgae (Nannochlropsis oculata) and the improvement of the subsequent culture. The results showed that struvite precipitation could improved the recovery rate of ammonia-nitrogen obviously, the recovery rate reached 98.8%. Two mediums were most suitable for the growth of N. oculata. The biomass productivity, protein content and carotenoid content of N. oculata in Group N1 acquired 1.0, 2.1, 6.0 times as much as the control group, respectively. While the biomass productivity, protein content and carotenoid content of N.oculata in Group N5 acquired 1.4, 1.6, 4.7 times as much as the control group, respectively. The subsequent algal suspension could be harvested again through ammonia-flocculation. Moreover more than 90% of flocculating rate was reached in 10 minutes. Therefore,struvite precipitation can effectively improve the subsequent culture.
Keywords:microalgae  struvite precitation  ammonia-flocculation  subsequent culture
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