人类巨细胞病毒HCMV UL49区域一个新转录本的发现

将人类巨细胞病毒HCMVTowne株接种至人包皮成纤维细胞(HFF)中,收集病毒感染后的细胞,抽提病毒RNA,运用RT-PCR技术扩增基因的序列,对PCR产物作TA克隆,重组体进行测序和生物信息学分析。结果表明,HCMV的UL49区域存在一个新转录本,暂命名为UL49X。将所获得的序列与HCMV基因组比对分析表明,UL49X序列为68783bp到78608bp处的基因片段,并发现从69194bp到71530bp处有个典型的内含子。

A new gene found in the UL49 region of HCMV

Incubated HCMV Towne to the well growing HFF cells;Collected the cells while the HFF cells appearing eytopathic effects;Extracted the HCMV RNA from the infected cells.Amplified the cDNA of the gene by RT-PCR.Finally,the production of RT-PCR were cloned into pMD18-T vectors and sequenced subsequently.And the sequence of UL49X was analyzed by bioinformatics.Our research show that, there is a new gene in the UL49 region of HCMV which we called it UL49X.The sequence of UL49X gene was from 68 783 bp to 78 608 bp,and we found that there was a intron between 69 194 bp and 71 530 bp.