香蕉MYB转录因子基因的电子克隆及生物信息学分析

采用电子克隆方法获得香蕉MYB基因.通过生物信息学方法,对此序列编码的蛋白质从氨基酸组成、理化性质、疏水性/亲水性、结构域及其空间结构等方面进行了预测和分析.结果表明:香蕉MYB基因编码序列全长1 266 bp,包含1 266 bp的开放阅读框,编码413个氨基酸,含有1个保守的MYB功能域、1个SANT保守结构域.在序列组成、理化性质、结构域及空间构象等方面,与小麦等其他植物的MYB转录因子具有高度的相似性.

In silico cloning and bioinformatics analysis of MYB gene of Musa paradisiaca

The banana MYB gene was cloned in silico based on the EST sequences from Unigene of NCBI.Some characters of MYB protein were analyzed and predicted by the tools of bioinformatics in the following aspects,including the composition of amino acid sequence, hydrophobicity or hydrophilicity, secondary and tertiary structureof protein and function. Results showed that the coding sequence of banana MYB was 1 266 bp, containing a 1 266bp ORF, which encoded 431 amino acids. As banana MYB contained one typical MYB domain, it could be concludedthat it belonged to R1-MYB subfamily. It was highly conservative compared with MYB from Triticum aestivum andother plant species in sequence composition, advanced structure and activity sites. The results could provide the basisfor MYB gene cloning in experiment and further research on its function.