| 苏云金芽孢杆菌aiiA基因的克隆及植物表达载体构建 |
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| 引用本文： | 欧阳乐军,沙月娥,郭耀权,邓玉金,曾富华.苏云金芽孢杆菌aiiA基因的克隆及植物表达载体构建[J].广东农业科学,2011,38(21). |
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| 作者姓名： | 欧阳乐军 沙月娥 郭耀权 邓玉金 曾富华 |
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| 作者单位： | 1. 湛江师范学院生命科学与技术学院,广东湛江524048;湖南农业大学生物科学与技术学院,湖南长沙410128
2. 湛江师范学院生命科学与技术学院,广东湛江,524048 |
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| 基金项目： | 国家星火计划项目(2011GA780067); 广东省自然科学基金(10152404801000005,10452404801004328); 广东省科技攻关项目(2010B020301011); 湛江市科技攻关项目(2011C3104010); 湛江师范学院校级项目(QL0913) |
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| 摘 要： | N-酰基高丝氨酸内酯(N-acyl-homoserine lactones,AHLs)是革兰氏阴性细菌致病过程中的关键信号分子,芽孢杆菌中广泛存在的aiiA基因编码AHL-Lactonase能够水解信号分子AHLs。利用PCR方法从苏云金芽孢杆菌中克隆了aiiA基因,序列分析表明该基因由753个碱基组成,编码含有250个氨基酸残基的蛋白质,核苷酸序列与已报道aiiA的同源性为89%～97%。将该基因连接到植物表达载体pCAMBIA1301中,成功构建了aiiA基因的植物表达载体pCAM-aiiA,为进一步通过转基因技术研究该基因的功能奠定了基础。
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| 关 键 词： | 苏云金芽孢杆菌 aiiA基因 群体感应 植物表达载体 |
Cloning and construction of plant expression vector of aiiA gene from Bacillus thuringiensis |
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| OUYANG Le-jun,SHA Yue-e,GUO Yao-quan,DENG Yu-jin,ZENG Fu-hua.Cloning and construction of plant expression vector of aiiA gene from Bacillus thuringiensis[J].Guangdong Agricultural Sciences,2011,38(21). |
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| Authors: | OUYANG Le-jun SHA Yue-e GUO Yao-quan DENG Yu-jin ZENG Fu-hua |
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| Institution: | OU YANG Le-jun1,2,SHA Yue-e1,GUO Yao-quan1,DENG Yu-jin1,ZENG Fu-hua1 (1.Life Science and Technology School,Zhanjiang Normal University,Zhanjiang 524048,China,2.College of Bioscience and Technology,Hunan Agricultural University,Changsha 410128,China) |
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| Abstract: | N-acyl-homoserine lactones(AHLs) is the crucial signal molecule when the gram-negative bacteria infects hose.The AHL-Lactonase expressed by aiiA gene which widespread in Bacillus thuringiensis,can hydrolyze AHLs.The study cloned the aiiA gene from Bacillus subtilis by PCR.The sequence analysis indicated that the clone was consisted of 751 nucleotides(nt),which coding 250 amino acids.The nucleotide sequence showed 89%~97% identities with those of the aiiA gene that have reported.A plant expression vector of ... |
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| Keywords: | Bacillus thuringiensis aiiA gene Quorum sensing plant expression vector |
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