翅萼石斛组织培养及快繁技术研究

以翅萼石斛蒴果为外植体,通过对其种子无菌萌发和原球茎诱导、原球茎增殖及丛生芽诱导、生根壮苗以及移栽驯化的研究,建立翅萼石斛的组培快繁技术体系。结果表明:翅萼石斛种子萌发和原球茎诱导的最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+蔗糖20 g/L;原球茎增殖和丛生芽诱导的最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+蔗糖20g/L+活性碳1.0g/L,接种45 d后增殖系数约5.8个、生长情况良好;生根壮苗的培养基配方为MS+6-BA 1.0 mg/L+KT 0.5 mg/L+NAA 0.2 mg/L+蔗糖30 g/L+香蕉泥100 g/L+活性碳1.0 g/L,生根率达100%,根系及幼苗长势良好。桂林地区移栽驯化宜在3~4月进行,选择水苔做基质,60 d后成活率为76.7%。

Tissue culture and rapid propagation of Dendrobium cariniferum

In order to establish a rapid propagation system for Dendrobium cariniferum Rchb. f., the immature seeds were selected as explants to study the suitable medium for for tissue culture, and mediums aseptic seed germination. Meanwhile, the appropriate multiplication conditions of protocorm and buds were selected subculture of protocom, and the rooting medium and transplanting matrix were also screened. The results showed that the medium of MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+ sucrose 20 g/L had a significant effect on seedlings germination and protocorm induction of D. cariniferum. The medium of MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+sucrose 20 g/L+activated carbon 1.0 g/L was suitable for protocorm proliferation and shoot induction, and the propagation coefficient reached 5.8 times after cultured 90 d. While the optimal medium for rooting was MS+6-BA 1.0 mg/L+KT 0.5 mg/L+NAA 0.2 mg/L+sucrose 30 g/L+banana mud 100 g/L+activated carbon 1.0 g/L, the rooting rate reached 100% and the roots had higher quality and quantity after cultured 90 d. The optimum time for the transplantation of root regeneration plants was March and April in Guilin area, and sphagna was suitable as media for transplanting and domestication, and the rooted plants had a survival rate of 76.7%.