| 长杆观赏甘蓝下胚轴离体再生研究 |
| |
| 引用本文： | 周岩,赵一鹏,蔡祖国.长杆观赏甘蓝下胚轴离体再生研究[J].广东农业科学,2009(9):68-70. |
| |
| 作者姓名： | 周岩 赵一鹏 蔡祖国 |
| |
| 作者单位： | 1. 河南科技学院生物技术系,河南,新乡,453003
2. 河南科技学院园艺系,河南,新乡,453003 |
| |
| 基金项目： | 教育部留学回国人员科研启动基金，河南科技学院高层次人才科研基金项目 |
| |
| 摘 要： | 以长杆观赏甘蓝(Brassica oleracea var.longata)实生幼苗下胚轴作为外植体,在附加细胞分裂素6-BA的MS培养基上,通过愈伤组织发生途径获得了再生植株,在研究中发现不同6-BA浓度(1、2、4、6 mg/L)对长杆观赏甘蓝不定芽形成有显著影响,其中在6-BA 4 mg/L培养基上的再生能力最强,每个外植体可再生出25.3个不定芽.此外,还对长杆观赏甘蓝离体培养的愈伤组织冉分化过程进行了组织学观察.
|
| 关 键 词： | 长杆观赏甘蓝 离体再生 组织学观察 |
Study on in vitro plant regeneration from hypocotyls of long-stem ornamental kale |
| |
| ZHOU Yan,ZHAO Yi-peng,CAI Zu-guo.Study on in vitro plant regeneration from hypocotyls of long-stem ornamental kale[J].Guangdong Agricultural Sciences,2009(9):68-70. |
| |
| Authors: | ZHOU Yan ZHAO Yi-peng CAI Zu-guo |
| |
| Institution: | ZHOU Yan1,ZHAO Yi-peng2,CAI Zu-guo2(1.Department of Biotechnology,Henan Institute of Science and Technology,Xinxiang 453003,China,2.Department of Horticulture,China) |
| |
| Abstract: | In vitro plant plantlet regeneration system was established from hypocotyls explant of long-stem ornamental kale(Brassica oleracea var.longata).The results showed that adventitious shoots could be differentiated from explant via callus on MS medium containing 6-BA,and 6-BA concentrations(1,2,4 and 6 mg/L) demonstrated significant affects on plantlet regeneration.It also exhibited that plantlet regeneration was achieved from explants cultured on MS medium supplemented with 4 mg/L 6-BA,e.g.25.3 adventious sho... |
| |
| Keywords: | Brassica oleracea var longata In vitro plant regeneration histological observation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《广东农业科学》浏览原始摘要信息 |
| 点击此处可从《广东农业科学》下载免费的PDF全文 |
|
