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| 动物源性激素DHEA对拟南芥植株根系生长发育的影响 | |
| 引用本文： | 杜冠魁,周思慧,朱丽娣,李栎,肖曼.动物源性激素DHEA对拟南芥植株根系生长发育的影响[J].广东农业科学,2013,40(3):29-31. |
| 作者姓名： | 杜冠魁 周思慧 朱丽娣 李栎 肖曼 |
| 作者单位： | 1. 海南医学院生物化学教研室,海南海口,571001 2. 海南医学院理学院,海南海口,571001 3. 海南医学院生物学教研室,海南海口,571001 |
| 基金项目： | 2011年海南医学院科研培育基金 |
| 摘 要： | 用DHEA处理拟南芥种子,观察经DHEA处理后拟南芥幼苗根系的生长状态,并研究其体内氧化还原状态的变化。结果表明:经DHEA处理后,拟南芥幼苗的主根缩短了20%,葡萄糖-6-磷酸脱氢酶(G6PD)酶活降低,过氧化氢(H2O2)、丙二醛(MDA)及还原性谷胱甘肽(GSH)含量升高,抗氧化酶总超氧化物歧化酶(T-SOD)和过氧化物酶(POD)活力均增强,但过氧化氢酶(CAT)活力下降。说明动物源性激素DHEA在植物中通过抑制G6PD的酶活,导致植株内的氧化还原状态发生变化,从而影响了主根的生长能力。 |
| 关 键 词： | DHEA 葡萄糖-6-磷酸脱氢酶 抑制 氧化状态 |
Effect of DHEA on the growth and development of root system of Arabidopsis seedling |
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| DU Guan-kui , ZHOU Si-hui , ZHU Li-di , LI Li , XIAO Man.Effect of DHEA on the growth and development of root system of Arabidopsis seedling[J].Guangdong Agricultural Sciences,2013,40(3):29-31. | |
| Authors: | DU Guan-kui ZHOU Si-hui ZHU Li-di LI Li XIAO Man |
| Institution: | 1(1.Department of Biochemistry,Hainan Medical College,Haikou 571001,China; 2.College of Science,Hainan Medical College,Haikou 571001,China; 3.Department of Biology,Hainan Medical College,Haikou 571001,China) |
| Abstract: | The Arabidopsis seeds were treated with DHEA to observe the alteration of root elongation and redox state of Arabidopsis seedings. The results showed that the primary root length was 80% of the control and the glucose-6-phosphate dehydrogenase activity decreased; the H2O2, MDA and GSH contents increased; the activity of T-SOD and POD was improved, although CAT activity reduced. These results suggested that the activity of G6PD was inhibited by DHEA, resulted in oxidative stress and inhibition of primary root growth in Arabidopsis seedlings. |
| Keywords: | DHEA glucose-6-phosphate dehydrogenase inhibition redox state |
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