| 不溶性草酸氧化酶的包埋及草酸快速检测方法研究 |
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| 引用本文： | 胡一鸿,张梦成,许春会.不溶性草酸氧化酶的包埋及草酸快速检测方法研究[J].广东农业科学,2011,38(9):107-109. |
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| 作者姓名： | 胡一鸿 张梦成 许春会 |
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| 作者单位： | 湖南人文科技学院生命科学系; |
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| 基金项目： | 湖南省教育厅重点项目(10A063); 湖南省科技计划项目(2009FJ3159); 湖南省大学生研究性学习和创新性实验计划项目(2010373) |
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| 摘 要： | 通过研究不溶性草酸氧化酶包埋方法和草酸快速检测方法,建立氧电极法测定草酸的便捷体系。以大麦麸皮为原料制备不溶性草酸氧化酶,采用5%的琼脂糖包埋,检测包埋酶的稳定性和抗离子干扰能力;以包埋酶为工具酶,通过氧电极法检测草酸含量。试验结果表明:包埋酶连续反应2 h酶活性下降低于5%,贮存30 d活性下降低于8%;草酸检测线性范围0.1～0.8mmol/L,外标法草酸回收率为83%～97%。用氧电极法与显色法测定小麦草酸含量,两种方法的测定值无显著性差异。但氧电极法测定耗时短,反应体系简单,易于推广应用。
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| 关 键 词： | 包埋 不溶性草酸氧化酶 大麦麸皮 氧电极 琼脂糖 |
Study on the methods of embedding insoluble oxalate oxidase and rapid determination of oxalic acid |
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| HU Yi-hong,ZHANG Meng-cheng,XU Chun-hui.Study on the methods of embedding insoluble oxalate oxidase and rapid determination of oxalic acid[J].Guangdong Agricultural Sciences,2011,38(9):107-109. |
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| Authors: | HU Yi-hong ZHANG Meng-cheng XU Chun-hui |
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| Institution: | HU Yi-hong,ZHANG Meng-cheng,XU Chun-hui(Department of Life Science,Hunan Institute of Humanities,Science and Technology,Loudi 417000,China) |
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| Abstract: | The methods of embedding insoluble oxalate oxidase and the rapid determination of oxalic acid were studied in order to establish a convenient oxalic acid assay system with oxygen electrodes.Insoluble oxalate oxidase was prepared from barley bran and embedded by 5% agarose gel.Its stability and resistance to ion disruption were determined.Oxalic acid content was also determined with oxygen electrodes method using the embedded enzyme.The results showed that the activity of the embedded enzyme decreased less t... |
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| Keywords: | embedding insoluble oxalate oxidase barley bran oxygen electrodes agarose |
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