| 大珠母贝组织蛋白酶D的cDNA克隆、序列特征分析及应激表达研究 |
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| 引用本文： | 潘俐玲,黄桂菊,喻达辉,成书营,王晓宁.大珠母贝组织蛋白酶D的cDNA克隆、序列特征分析及应激表达研究[J].广东农业科学,2011,38(10):4-9. |
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| 作者姓名： | 潘俐玲 黄桂菊 喻达辉 成书营 王晓宁 |
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| 作者单位： | 1. 农业部海水养殖生态与质量控制重点开放实验室,中国水产科学研究院南海水产研究所,广东广州510300;上海海洋大学水产与生命学院,上海201306
2. 农业部海水养殖生态与质量控制重点开放实验室,中国水产科学研究院南海水产研究所,广东广州510300 |
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| 基金项目： | 国家“863”计划项目(2006AA10A415); 国家现代农业产业技术体系建设专项(CARS-48); 广东省科技推广项目(A200701C02,A200899C04,A200900A07); 中央级公益性科研院所基本科研业务费专项(2007TS07,2009YD02) |
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| 摘 要： | 通过同源克隆方法和cDNA末端快速扩增技术(RACE),获得了大珠母贝(Pinctada maxima)组织蛋白酶D基因(命名为pmCTSD)的cDNA全长序列1 742 bp,其中5'非翻译区(UTR)38 bp,3'UTR 534 bp,开放阅读框1 170 bp,编码390个氨基酸,分子量约为41.9 ku,等电...
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| 关 键 词： | 大珠母贝 组织蛋白酶D 序列特征分析 哈维式弧菌刺激 组织表达 |
Molecular cloning and characterization of cathepsin D in pearl oyster Pinctada maxima and its tissue expression profiles under Vibrio harveyi challenge |
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| PAN Li-ling,HUANG Gui-ju,YU Da-hui,CHENG Shu-ying,WANG Xiao-ning.Molecular cloning and characterization of cathepsin D in pearl oyster Pinctada maxima and its tissue expression profiles under Vibrio harveyi challenge[J].Guangdong Agricultural Sciences,2011,38(10):4-9. |
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| Authors: | PAN Li-ling HUANG Gui-ju YU Da-hui CHENG Shu-ying WANG Xiao-ning |
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| Institution: | PAN Li-ling1,2,HUANG Gui-ju1,YU Da-hui1,CHENG Shu-ying1,WANG Xiao-ning1,2(1.Key Lab of Mariculture,Ecology and Quality Control,Ministry of Agriculture,Division of Aquaculture and Biotechnology/South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China,2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | The full length of pearl oyster Pinctada maxima cathepsin D(named as pmCTSD) was determined for the first time through homologous cloning and rapid amplification of cDNA ends(RACEs) approaches.The full length cDNA contained a 5' UTR of 38 bp,an open reading frame(ORF) of 1 170 bp encoding 390 amino acids and a 3' UTR of 534 bp.The deduced amino acid residues comprised a signal peptide of 18 aa,a pro-sequence of 29 aa and a mature protein of 343 aa,with an estimated isoelectric point of 7.57 and molecular ma... |
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| Keywords: | cathepsin D sequence analysis Pinctada maxima vibrio challenge tissue expression |
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