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两株红树林真菌共培养液抗植物病菌成分研究
引用本文:丁唯嘉,黎荣棠,李春远,佘志刚,林永成.两株红树林真菌共培养液抗植物病菌成分研究[J].广东农业科学,2011,38(9):72-74,77.
作者姓名:丁唯嘉  黎荣棠  李春远  佘志刚  林永成
作者单位:华南农业大学理学院生物材料研究所;中山大学化学与化学工程学院;
基金项目:广东省自然科学基金(9451064201003751); 华南农业大学“211工程”三期重点建设项目(2009B010100001)
摘    要:从南海红树林内生真菌E33和K38混合发酵液提取物中寻找有抗植物病原菌活性的化合物,利用硅胶柱层析共分离得到6个化合物,用波谱技术分别鉴定后,确定为对苯二甲酸二甲酯、4-甲氧酰基苯甲酸乙二醇酯、4-甲基-2-甲酰基-3,5-二羟基苯甲酸乙酯、rubramin、对羟基乙酰苯乙胺和尿嘧啶。4-甲基-2甲酰基-3,5-=羟基苯甲酸乙酯首次以天然产物的形式被发现,并首次报道了其2D-NMR数据,生测结果表明其对小麦赤霉、大豆疫霉具有中等的抗菌活性。

关 键 词:海洋真菌  红树内生真菌  共培养  抗真菌作用  

Study on chemical constitents with antifungal activity against plant pathogens of the co-culture broth of two mangrove fungi
DING Wei-jia,LI Rong-tang,LI Chun-yuan,SHE Zhi-gang,LIN Yong-cheng.Study on chemical constitents with antifungal activity against plant pathogens of the co-culture broth of two mangrove fungi[J].Guangdong Agricultural Sciences,2011,38(9):72-74,77.
Authors:DING Wei-jia  LI Rong-tang  LI Chun-yuan  SHE Zhi-gang  LIN Yong-cheng
Institution:DING Wei-jia1,LI Rong-tang1,LI Chun-yuan1,SHE Zhi-gang2,LIN Yong-cheng2(1.Institute of Biomaterial,College of Science,South China Agricultural University,Guangzhou 510642,China,2.School of Chemistry and Chemical Engineering,Sun Yat-sen University,Guangzhou 510275,China)
Abstract:Six compounds were isolated from the mixed fermention liquid of two mangrove fungi(No.E33 and K38) by silica gel column chromatography.Their structures were determined to be dimethyl terephthalate,terephthalic acid 2-hydroxyethyl methyl ester,ethyl 2-formyl-3,5-dihydroxy-4-methylbenzoate,rubramin,p-acetamidophenol and urail on the basis of comprehensive spectroscopic analysis.Compounds 3 was first found as a natural product,compound 2~4 were isolated from marine fungus for the first time.The techque of 2D-N...
Keywords:marine fungus  mangrove fungus  co-culture  antifungal activity  
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