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| 苹果多酚体内外抗甲型流感病毒作用 | |
| 引用本文： | 李润峰,何蓉蓉,栗原博,陈建新.苹果多酚体内外抗甲型流感病毒作用[J].广东农业科学,2011,38(14). |
| 作者姓名： | 李润峰 何蓉蓉 栗原博 陈建新 |
| 作者单位： | 1. 华南农业大学兽医学院,广东省兽药研制与安全评价重点实验室,广东广州510642 2. 广州医学院第一附属医院呼吸疾病国家重点实验室,广东广州,510230 3. 暨南大学中药及天然药物研究所,广东广州,510632 |
| 基金项目： | 国家自然科学基金(30972217); 广东省教育部产学研结合项目(2009B090300331); 广东省科技计划重点项目(2009B020600001) |
| 摘 要： | 体外试验利用甲型流感病毒感染狗肾细胞(MDCK)致细胞病变(CPE)模型评价苹果多酚对流感病毒复制的抑制作用;体内试验利用小鼠感染流感病毒致鼠肺适应株模型,以死亡保护率和生命延长率等为指标,评价苹果多酚体内抗流感病毒作用。利用体外不同作用模式试验、不同作用时间点试验和血凝抑制试验(HI)研究苹果多酚抗流感病毒作用机制。研究结果表明,苹果多酚对甲型H9N2、H3N2和H1N1 3种亚型流感病毒引起的细胞病变表现出不同程度的抑制作用,50%抑制浓度(IC50)分别是0.012、0.0042、0.003 mg/mL。200 mg/(kg.d)剂量苹果多酚对H1N1亚型流感病毒感染小鼠有明显的保护作用和延长生命率的作用(P<0.05)。苹果多酚对3种亚型流感病毒的血凝抑制最小浓度(MIC)在0.25～0.5 mg/mL范围之间。对病毒复制周期的吸附和穿入的抑制作用是其主要作用机制。说明苹果多酚在体内外均具有显著的抗流感病毒作用。 |
| 关 键 词： | 苹果多酚 流感病毒 抗流感病毒 小鼠 |
Study on anti-influenza virus activity of apple polyphenols in vivo and in vitro |
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| LI Run-feng,HE Rong-rong,Hiroshi Kurihara,CHEN Jian-xin.Study on anti-influenza virus activity of apple polyphenols in vivo and in vitro[J].Guangdong Agricultural Sciences,2011,38(14). | |
| Authors: | LI Run-feng HE Rong-rong Hiroshi Kurihara CHEN Jian-xin |
| Institution: | LI Run-feng1,HE Rong-rong2,Hiroshi Kurihara3,CHEN Jian-xin1(1.College of Veterinary Medicine,South China Agricultural University/The Guangdong Provincial Key Laboratory of Veterinary Drugs'Research and Safety Evaluation,Guangzhou 510642,China,2.State Key Laboratory of Respiratory Disease,Guangzhou Institute of Respiratory Disease,Guangzhou 510230,3.Institute of Traditional Chinese Medicine and Natural Products,Jinan University,Guangzhou 510632,China) |
| Abstract: | Viral cytopathic effect(CPE) was used to evaluate anti-influenza activity of apple polyphenols in vitro.Mouse infection model was used with life-protection rate as parameters to indicate inhibitory effect of apple polyphenols on influenza virus in vivo.Mechanism of action of apple polyphenols in detail was studied by action modes study,time of addition study and HI assay.The results showed that apple polyphenols exhibited inhibitory effect at various degree against a spectrum of influenza A(H9N2,H3N2 and H1... |
| Keywords: | apple polyphenols influenza virus anti-influenza virus mouse |
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