| 花生幼叶丛生芽的诱导和高效再生体系的建立 |
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| 引用本文： | 李艳,李少雄,周桂元,洪彦斌,林坤耀,梁炫强.花生幼叶丛生芽的诱导和高效再生体系的建立[J].广东农业科学,2006(7):14-16. |
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| 作者姓名： | 李艳 李少雄 周桂元 洪彦斌 林坤耀 梁炫强 |
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| 作者单位： | 广东省农科院作物研究所,广东,广州,510640 |
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| 基金项目： | 广东省农业科技攻关项目;广东省农业科学院科技攻关项目 |
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| 摘 要： | 以花生种子无菌条件下萌发4天、7天、10天、27天的幼叶作外植体,分别接种于加有不同激素配方的芽诱导培养基上,1周后叶片伤口处膨大并有黄绿色愈伤组织出现,3周左右可以看到丛生芽点的出现,芽诱导率达85%以上;继代培养4周后转至高浓度BA(10 mg/L)的MS培养基上促进芽伸长;当小苗长至3 cm左右时,移入NAA(1 mg/L)的MS培养基上诱导生根,获得完整的再生植株,再生率可以达到80%以上。
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| 关 键 词： | 花生 离体培养 诱导 丛生芽 外植体 再生体系 |
| 文章编号： | 1004-874X(2006)07-0014-03 |
| 收稿时间： | 2006-02-23 |
| 修稿时间： | 2006年2月23日 |
Preliminary report on the in vitro culture of shoot induction from leaflets of peanut (Arachis hypogeae L.) |
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| LI Yan,LI Shao-xiong,ZHOU Gui-yuan,HONG Yan-bin,LIN Kun-yao,LIANG Xuan-qiang.Preliminary report on the in vitro culture of shoot induction from leaflets of peanut (Arachis hypogeae L.)[J].Guangdong Agricultural Sciences,2006(7):14-16. |
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| Authors: | LI Yan LI Shao-xiong ZHOU Gui-yuan HONG Yan-bin LIN Kun-yao LIANG Xuan-qiang |
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| Institution: | Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China |
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| Abstract: | The shoots were induced in vitro from peanut leaflets at 4 days,7 days,10 days and 27 days old on MS medium with different plant growth regulator.The callus formed after 1 week,and the shoots were observed after 3 weeks,the highest rate of organgenesis was up to 85%.The shoots grew further in MS medium with high BA concentration(10 mg/L) in about 4 weeks.At last the regeneration peanut seedling developed rooting on MS medium with NAA(1 mg/L).The rate of regeneration was up to 80%. |
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| Keywords: | peanut in vitro culture induction adventitious shoots explants regeneration system |
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