马铃薯衰老相关基因的电子克隆与生物信息学分析

利用电子克隆获得马铃薯衰老相关基因(StSAG)的cDNA序列,并对其进行生物信息学分析.结果表明,马铃薯衰老相关基因的cDNA序列全长1 062 bp,包含一个561 bp的开放阅读框,编码186个氨基酸;StSAG的等电点为7.20,为亲水叶绿体蛋白,无信号肽,有12个磷酸化位点,在N末端有一个较短的伸展肽段.StSAG与茄科植物烟草亲缘关系最近.

Electronic cloning and bioinformatical analysis of senescence-associated genes from Solanum tuberosum

The cDNA sequence of senescence-associated gene (SAG) from Solanum tuberosum was acquired by electronic cloning. By using the methods of bioinformatics, we analyzed some characteristics of its encoded protein sequence. The results showed that the full-length of StSAG was 1 062 bp, which contained a 561 bp open reading frame encoding 186 amino acids. The isoelectric point of StSAG was 7.20, as a hydrophilic chloroplast protein without signal peptide. StSAG had 12 phosphorylation sites and a short extension peptide in the N terminal. The phylogenetic relationship between StSAG and Solanaceae plant was the closest.