| 大孔吸附树脂分离提取发酵液中伊枯草菌素A的研究 |
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| 引用本文： | 张婷,杨杰,周金燕,谭红.大孔吸附树脂分离提取发酵液中伊枯草菌素A的研究[J].广东农业科学,2012,39(8):94-96. |
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| 作者姓名： | 张婷 杨杰 周金燕 谭红 |
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| 作者单位： | 1. 中国科学院成都生物研究所,四川成都610041;中国科学院研究生院,北京100039
2. 中国科学院成都生物研究所,四川成都,610041 |
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| 基金项目： | 国家科技支撑计划项目，中国科学院知识创新工程重要方向项目 |
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| 摘 要： | 研究了利用大孔吸附树脂从发酵液中提取伊枯草菌素A的工艺条件。对比了不同极性大孔树脂对发酵液中伊枯草菌素A的分离效果,选择DM301型树脂作为吸附剂进行优化试验。结果表明,在25℃时,每升发酵液吸附剂用量为80 g DM301型树脂时,树脂对发酵液中伊枯草菌素A的吸附率可达100%。滤去吸附残液,采用无水乙醇解吸,每克DM301树脂解吸剂用量为10 mL时,静态解吸率达90%以上。动态吸附试验表明,发酵液的最佳上样量为6倍柱床体积,动态解吸流出液活性高度集中。该工艺简单、易操作,适用于工业化生产。
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| 关 键 词： | 伊枯草菌素A 大孔吸附树脂 吸附 解吸 抑菌圈直径 |
Separation and extraction of Iturin A from fermentation broth with macroporous resin |
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| ZHANG Ting
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YANG Jie
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ZHOU Jin-yan
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TAN Hong.Separation and extraction of Iturin A from fermentation broth with macroporous resin[J].Guangdong Agricultural Sciences,2012,39(8):94-96. |
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| Authors: | ZHANG Ting YANG Jie ZHOU Jin-yan TAN Hong |
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| Institution: | 1(1.Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China; 2.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China) |
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| Abstract: | A new method for separation and extraction of Iturin A from fermentation broth with macroporous resin was carried out in this work.Among six macroporous resins,DM-301 resin was the best resin with high adsorption capacity and deabsorption capacity in static tests.The results showed that static adsorption rate of DM-301 resin can amount to 100%,with 80 g resin per liter fermentation broth in 25℃.Static desorption rate can amount to 90% by ethanol,with 10 mL ethanol per gram resins.Dynamic adsorption tests indicated that Iturin A started to leak out when the sample was 6 BV.The activity of dynamic desorption outflow liquid was highly concentrated.The process is easy to operate and is fit for industrial production. |
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| Keywords: | Iturin A macroporous resin adsorption deabsorption inhibition zone |
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