20 个烟草品种 TMV 抗性分析及抗病浸种药剂筛选

通过种子发芽、幼苗生长特征分析对20个烟草品种苗期抗TMV特性进行初步鉴定、分类,分别选择抗性品种云烟201和感性品种MSK326为材料,研究不同抗性品种经TMV病毒浸染后的幼苗生长情况,并测定各生理生化指标.采用不同药剂对2个品种种子浸种处理后比较种子抗病效果,结果表明药剂处理可以提高烟草种子及幼苗抗病性.综合各项指标,采用1g/L甲基托布津溶液能够显著降低感性品种MSK326的发病率和发病指数,同时显著提高该品种的叶绿素a、b含量和过氧化酶活性.

TMV resistance analysis of 20 tobacco cultivars and screening of resistant soaking reagents

In this study, TMV resistant characteristics of 20 tobacco cultivars were preliminarily identified and classified based on analyzing the seed germination and seedling growth. Then, Yunyan 201 was chosen as tolerant cultivar and MSK326 was chosen as the sensitive one to be contaminated by TMV virus and then the seedling growth was investigted. The physiological and biochemical indexes were also tested. The seeds of those two cultivars were soaked with different reagents to compare the tolerant effects of TMV, which showed that soaking with reagents could increase the TMV tolerance of tobacco seeds and seedlings. In summary, using 1 g/L thiophanate methyl solution could significantly reduce the morbidity rate and morbidity index of MSK326, and increase its chlorophyll a and b content and peroxidase activity.