| SYBR-Green实时荧光PCR检测转基因番木瓜 |
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| 引用本文： | 刘建越,邓欣,康林,余道坚,高必达,陈冬美.SYBR-Green实时荧光PCR检测转基因番木瓜[J].湖南农业大学学报(自然科学版),2006,32(4):371-374. |
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| 作者姓名： | 刘建越 邓欣 康林 余道坚 高必达 陈冬美 |
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| 作者单位： | 1. 湖南农业大学,生物安全科学技术学院,湖南,长沙,410128
2. 深圳出入境检验检疫局,动植物检疫技术中心,广东,深圳,518010 |
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| 基金项目： | 广东省深圳市出入境检验检疫局资助项目 |
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| 摘 要： | 分别以RBCL基因和PRsV-cP基因、PRSV-RP基因为内源基因和目标基因，设计了3对特异性引物，对转基因番木瓜进行了SYBRGreen实时荧光PCR检测．结果表明，RBCL基因引物对所有供试样品成功扩增，0值为15～16，PRSV-CP引物对转PRSV-CP基因番木瓜样品成功扩增，Ct值为20～25，PRSV-RP引物对转PRSV-RP基因番木瓜样品成功扩增，Ct值为21～22．运用熔解曲线进行产物分析，验证了试验结果的特异性和准确性．
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| 关 键 词： | 转基因番木瓜 番木瓜环斑病毒 SYBR-Green 实时荧光PCR 检测 |
| 文章编号： | 1007-1032(2006)04-0371-04 |
| 修稿时间： | 2006年3月20日 |
Real-Time Fluorescent SYBR-Green PCR Detect the Genetically Modified Papaya |
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| LIU Jian-yue,DENG Xin,KANG Lin,YU Dao-jian,GAO Bi-da,CHEN Dong-mei.Real-Time Fluorescent SYBR-Green PCR Detect the Genetically Modified Papaya[J].Journal of Hunan Agricultural University,2006,32(4):371-374. |
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| Authors: | LIU Jian-yue DENG Xin KANG Lin YU Dao-jian GAO Bi-da CHEN Dong-mei |
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| Abstract: | This thesis studied on the detection of genetically modified papaya by real-time fluorescent SYBR Green PCR using the ribulose bisphosphate carboxylase/oxygenase large subunit as the endogenesis reference gene and the papaya ringspot virus coat protein,replicating protein gene as the target gene.The results showed that RBCL primers can successfully amplify all the experimental samples,Ct value was 15~16;PRSV-CP primers can successfully amplify the experimental samples which contained PRSV-CP gene,Ct value was 20~25;PRSV-RP primers can successfully amplify the experimental samples which contained PRSV-RP gene,Ct value was 21~22.The amplified products were analyzed by the melting sequence allis,it also proved that the experiment results were differential and veracious. |
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| Keywords: | genetically modified papaya papaya ringspot virus real-time fluorescent SYBR-Green PCR detection |
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