绵羊Oct4基因启动子序列的克隆及其与猪和牛序列的比较分析

为探明绵羊Oct4基因启动子的结构特点,用PCR方法克隆获得了绵羊Oct4基因启动子,并对绵羊、牛和猪Oct4基因的上游调控序列进行对比分析。结果显示,成功扩增获得长度为2 951 bp的绵羊Oct4基因启动子;绵羊、牛和猪Oct4基因的上游调控序列均含有4个保守区域(CR1–CR4),且4个保守区域在3个物种间具有高度同源性;CR1区域富含GC碱基对,且序列上的Sp1/Sp3位点与激素反应元件HRE在3个物种中具有100%的同源性;绵羊和牛、猪的上游调控序列富含GC,并存在大量的CCC(A/T)CCC位点。

Cloning promoter Oct4 from sheep and its sequence comparison with bovine and porcine upstream promoter sequences

To explore the structural characteristics of gene promoterOct4in sheep, 5' upstream regulatory regions of ovine geneOct4were cloned using PCR, and the comparative alignment analysis among sheep, cow and pig was conducted as well. The results showed that the promoter was 2 951 bp in length. There were four conserved regions (CR 1 to 4) in theupstream regulatory sequence of geneOct4in sheep, cow and pig, and the four regions presented in high homology among the three species. A putative Sp1/Sp3 binding site and the overlapping hormone responsive element (HRE) in CR 1 region were identical to each other among the three species, moreover, there were rich in GC and a high number of CCC (A/T) CCC motifs in the upstream regions. The research could provide reference for futher understanding the gene Oct4 and facilitate the relevant research on sheep.