| 大孔吸附树脂分离茶儿茶素和咖啡因的研究 |
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| 引用本文： | 龚雨顺,刘仲华,黄建安,王坤波,刘硕谦,张盛.大孔吸附树脂分离茶儿茶素和咖啡因的研究[J].湖南农业大学学报(自然科学版),2005,31(1):50-52. |
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| 作者姓名： | 龚雨顺 刘仲华 黄建安 王坤波 刘硕谦 张盛 |
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| 作者单位： | 湖南农业大学,天然产物研究中心,湖南,长沙,410128 |
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| 基金项目： | 国家计委攻关项目(计高技 2284 号,2002) |
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| 摘 要： | 为克服有毒溶济萃取法和金属离子沉淀法分离儿茶素和咖啡因造成环境污染和操作有安全隐患等缺陷，以粗品茶多酚为原料，研究了利用大孔吸附树脂柱色谱分离制备儿茶素和咖啡因的可行性．结果表明，大孔吸附树脂对儿茶素和咖啡因的吸附和解吸性能良好，通过梯度洗脱，可对儿茶素和咖啡因进行分离，并能制得不同规格的儿茶素产品和粗品咖啡因，其中高EGCg含量(66．95％)的高纯儿茶素产品GTC-90制率达46．47％，咖啡因低于0．5％．
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| 关 键 词： | 大孔吸附树脂 分离 制备 儿茶素 咖啡因 |
| 文章编号： | 1007-1032(2005)01-0050-03 |
| 修稿时间： | 2004年5月19日 |
Studies on the Isolation of Tea Catechins and Caffeine with the Macroporous Adsorption Resin |
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| GONG Yu-shun,LIU Zhong-hua,HUANG Jian-an,WANG Kun-bo,LIU Shuo-qian,ZHANG Sheng.Studies on the Isolation of Tea Catechins and Caffeine with the Macroporous Adsorption Resin[J].Journal of Hunan Agricultural University,2005,31(1):50-52. |
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| Authors: | GONG Yu-shun LIU Zhong-hua HUANG Jian-an WANG Kun-bo LIU Shuo-qian ZHANG Sheng |
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| Abstract: | Column chromatography was adopted to isolate catechins and caffeine in crude polyphenols with the macroporous adsorption resin. Results showed that the capability of adsorption and desorption was very good between the resin and polyphenols,catechins and caffeine could be isolated with gradient elution,a series products of catehins and crude caffeine were gained. Furthermore,the product of high-pure catechins(GTC-90) could be obtained in which EGCg occupied 66.95%,and caffine no more than 0.5%. |
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| Keywords: | macroporous adsorption resin isolation preparation catechins caffeine |
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