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| 红海榄根部总蛋白质提取方法的改进和双向电泳体系的建立 | |
| 引用本文： | 范吉星,邓用川,黄惜,林栖凤,罗越华.红海榄根部总蛋白质提取方法的改进和双向电泳体系的建立[J].湖南农业大学学报(自然科学版),2008,34(5). |
| 作者姓名： | 范吉星 邓用川 黄惜 林栖凤 罗越华 |
| 作者单位： | 1. 海南大学,农学院,海南,海口,570228 2. 海南省耐盐作物生物技术重点实验室,海南,海口,570228 3. 海南大学,农学院,海南,海口,570228;海南省耐盐作物生物技术重点实验室,海南,海口,570228 |
| 基金项目： | 海南省重点科技项目，海口市农业三项科技项目，海南省耐盐作物生物技术重点实验室开放基金 |
| 摘 要： | 分别采用TCA-丙酮沉淀法、酚法、尿素法、周涵韬法和笔者建立的酚改良法,提取红海榄根部总蛋白质,并进行了SDS-PAGE分析和双向电泳体系的优化.结果表明,酚改良法提取红海榄根部总蛋白质的纯度最高,质量最好,为建立双向电泳分析体系提供了较为理想的蛋白样品,获得了良好的双向电泳图谱.该结果为盐生植物,尤其是盐生木本植物,建立了一套可靠的蛋白提取和双向电泳方法. |
| 关 键 词： | 红海榄 蛋白质 双向电泳 |
Improvement of protein extraction method from Rhizophora stylosa root and establishment of two-dimensional electrophoresis |
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| FAN Ji-xing,DENG Yong-chuan,HUANG Xi,LIN Qi-feng,LUO Yue-hua.Improvement of protein extraction method from Rhizophora stylosa root and establishment of two-dimensional electrophoresis[J].Journal of Hunan Agricultural University,2008,34(5). | |
| Authors: | FAN Ji-xing DENG Yong-chuan HUANG Xi LIN Qi-feng LUO Yue-hua |
| Institution: | FAN Ji-xing1,DENG Yong-chuan2,HUANG Xi2,LIN Qi-feng2,LUO Yue-hua1,2 (1.College of Agronomy,Hainan University,Haikou 570228,China,2.Hainan Provincial Key Laboratory of Biotechnology for Salt-tolerant Crop,China) |
| Abstract: | Several methods of plant tissue protein extraction (TCA-acetone precipitation method,phenol method,urea method,Zhou Han-tao described method,and phenol method improved by the authors) were applied to extract total protein from Rhizophora stylosa root,and then after SDS-PAGE, a subsequent 2-DE analysis was carried out with the optimized conditions for 2-DE . The results indicated that,using the modified phenol method, by the authors more qualified protein with higher purification could be obtained compared w... |
| Keywords: | Rhizophora stylosa protein two-dimensional electrophoresis(2-DE) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |