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草鱼肠道抗菌肽对细菌形态结构的影响及理化性质研究
引用本文:陈韬,黄平,彭宽,黄小西,章怀云,张东裔.草鱼肠道抗菌肽对细菌形态结构的影响及理化性质研究[J].湖南农业大学学报(自然科学版),2010,36(1):69-72.
作者姓名:陈韬  黄平  彭宽  黄小西  章怀云  张东裔
作者单位:1. 中南林业科技大学生命科学与技术学院,湖南,长沙410004
2. 中南林业科技大学生物技术开放性中心实验室,湖南,长沙410004;湖南农业大学,动物医学院,湖南,长沙,410128
3. 中南林业科技大学生物技术开放性中心实验室,湖南,长沙410004
4. 中南林业科技大学生物技术开放性中心实验室,湖南,长沙410004;长沙学院,生物技术研究所,湖南,长沙,410003
基金项目:湖南省教育厅优秀青年基金项目,中南林业科技大学青年科学基金项目 
摘    要:通过扫描电子显微镜观察草鱼肠道抗菌肽处理后的大肠杆菌、金色葡萄球菌、嗜水气单胞菌的形态结构的变化.结果显示,经过抗菌肽处理后的细菌形态发生变化,菌体严重变形,表面变得粗糙,出现皱褶,胞膜破裂.对草鱼肠道抗菌肽的理化性质的研究结果显示,草鱼肠道抗菌肽具有很好的热稳定性,耐胰蛋白酶特性.

关 键 词:肠道抗菌肽  扫描电子显微镜  草鱼  理化性质
收稿时间:2009/9/1 0:00:00
修稿时间:2009/9/1 0:00:00

Effects of antimicrobial peptide from intestine of grass carp on morphology of bacteria and its physicochemical properties
chentao,huang ping,peng kuan,huang xiaoxi,zhang huaiyun and zhang dongyi.Effects of antimicrobial peptide from intestine of grass carp on morphology of bacteria and its physicochemical properties[J].Journal of Hunan Agricultural University,2010,36(1):69-72.
Authors:chentao  huang ping  peng kuan  huang xiaoxi  zhang huaiyun and zhang dongyi
Institution:Hunan Agricultural University,Central south university of forestry and technology,Central south university of forestry and technology,Central south university of forestry and technology,Central south university of forestry and technology,Central south university of forestry and technology
Abstract:Morphological changes of bacteria,E.coli,S.aureus,A.hydrophila,treated with antimicrobial peptide were observed by scanning electron microscopy.The results showed that the cells deformed severely,the surface of bacteria became rough and rumply,and the cytomembrane was ruptured after treated by antimicrobial peptide from intestine of grass carp.Moreover,research on its physicochemical properties showed antimicrobial peptide from the intestine of grass carp had an excellent thermal stability and strong resistance to trypsin activity.
Keywords:antimicrobial peptide of intestine  SEM  grass carp  physicochemical properties
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