| 毒麦属6个种的rDNA ITS序列测定及分析 |
| |
| 引用本文： | 张姮,康林,高必达,陈冬美.毒麦属6个种的rDNA ITS序列测定及分析[J].湖南农业大学学报(自然科学版),2009,35(1). |
| |
| 作者姓名： | 张姮 康林 高必达 陈冬美 |
| |
| 作者单位： | 1. 湖南农业大学,生物安全科学技术学院,湖南,长沙,410128
2. 深圳出入境检验检疫局,动植物检疫技术中心,广东,深圳,518010 |
| |
| 基金项目： | 国家质量检验检疫总局项目 |
| |
| 摘 要： | 提取毒麦属6个种的样品DNA,分别采用PCR产物直接测序和克隆测序2种方法,对毒麦属6个种rDNA的ITS区(包括ITS-1,5.8 S rDNA和ITS-2)进行序列测定.结果表明,毒麦属植物ITS序列总长度约为647 bp,其中ITS-1,5.8 S rDNA和ITS-2分别有16、7和7个变异位点.采用NJ法建立的系统发育树与形态学分类基本相符.
|
| 关 键 词： | 毒麦属 ITS序列 |
Sequence determination and analysis of the ITS region of nuclear ribosomal DNA in six species of Lolium |
| |
| ZHANG Heng,KANG Lin,GAO Bida,CHEN Dong-mei.Sequence determination and analysis of the ITS region of nuclear ribosomal DNA in six species of Lolium[J].Journal of Hunan Agricultural University,2009,35(1). |
| |
| Authors: | ZHANG Heng KANG Lin GAO Bida CHEN Dong-mei |
| |
| Institution: | 1.College of Bio-Safety Science and Technology;HNAU;Changsha 410128;China;2.Shenzhen Entry-Exit Inspection and Quarantine Bureau;Shenzhen;Guangdong 518010;China |
| |
| Abstract: | DNAs of 6 samples from Lolium were extracted. The sequence of rDNA ITS region (including ITS-1,5.8 S rDNA and ITS-2) were determined from 6 species of Lolium by the direct or clone sequencing of PCR products. The results showed that the full length of Lolium ITS was about 647 bp,The numbers of variable sites were 16,7 and 7 in ITS-1,5.8 S and ITS-2,respectively. The phylogenetic tree was based on NJ method corresponds with the morphological classification,which provided an important molecular basis for the ... |
| |
| Keywords: | rDNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
