|
|||||
|
|
| 黑曲霉AF-1固态发酵生产生淀粉酶的条件优化 | |
| 引用本文： | 苏小军,熊兴耀,谭兴和,李清明,罗时,易锦琼.黑曲霉AF-1固态发酵生产生淀粉酶的条件优化[J].湖南农业大学学报(自然科学版),2009,35(2). |
| 作者姓名： | 苏小军 熊兴耀 谭兴和 李清明 罗时 易锦琼 |
| 作者单位： | 1. 湖南农业大学作物种质创新与资源利用国家重点实验室培育基地,湖南,长沙,410128;湖南农业大学园艺园林学院,湖南,长沙,410128 2. 湖南农业大学作物种质创新与资源利用国家重点实验室培育基地,湖南,长沙,410128;食品科学技术学院,湖南,长沙,410128 3. 食品科学技术学院,湖南,长沙,410128 4. 湖南农业大学园艺园林学院,湖南,长沙,410128 |
| 摘 要： | 为增强黑曲霉AF-1产生生淀粉酶的能力,对其固态发酵产酶条件进行了优化.采用单因素试验分别筛选出马铃薯粉、豆粕粉、FeSO4为最适碳源、氮源和无机盐;通过Plackett-Burman设计对影响产酶条件的12个相关因素进行效应评价,筛选出具有显著效应的培养基豆粕粉、麸皮含量和温度3个因素;利用最陡爬坡试验逼近以上3个因素的最大响应区域,采用响应面中心组合设计对3个因素进行优化,得出培养基豆粕粉含量、麸皮添加量和温度的最佳值分别为11.46%、17.41 g和26.26 ℃.优化后的酶活力(204 U/mL)比初始酶活力(42 U/mL)提高了3.85倍. |
| 关 键 词： | 黑霉AF-1 固态发酵 生淀粉酶 |
Optimization of raw-starch-hydrolysing amylase production by Aspergillus niger AF-1 in solid state fermentation |
|
| SU Xiao-jun,XIONG Xing-yao,TAN Xing-he,LI Qing-ming,LUO Shi,YI Jin-qiong.Optimization of raw-starch-hydrolysing amylase production by Aspergillus niger AF-1 in solid state fermentation[J].Journal of Hunan Agricultural University,2009,35(2). | |
| Authors: | SU Xiao-jun XIONG Xing-yao TAN Xing-he LI Qing-ming LUO Shi YI Jin-qiong |
| Institution: | a.Pre-State Key Laboratory for Germplasm Innovation and Utilization of Crop;b.College of Horticulture and Landscape;c.College of Food Science and Technology;HNAU;Changsha 410128;China |
| Abstract: | Solid state fermentation was optimized for improvement of raw-starch-hydrolysing amylase production by Aspergillus niger AF-1.Potato flour,soybean powder,and FeSO4 were screened out as the most suitable carbon source,nitrogen source and inorganic salt source,respectively.Based on these,screening methodology Plackett-Burman design was used to evaluate the effects of twelve factors related to amylase production and three statistically significant factors soybean powder,temperature,bran were selected.The path ... |
| Keywords: | Aspergillus niger AF-1 solid state fermentation raw-starch-hydrolysing amylase |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |