Fe3O4纳米颗粒细胞毒性的研究

为了研究Fe3O4纳米颗粒在突变细胞系的中的毒性问题，采用不同浓度Fe3O4纳米颗粒处理含有核苷酸剪切修复基因XPF突变的原代XPF细胞与HeLa细胞.细胞集落形成实验证实了Fe3O4纳米颗粒对XPF细胞的生长有显著的抑制作用，表现出了一定的细胞毒性，说明Fe3O4纳米颗粒不适合应用于有核苷酸剪切修复基因突变的人群.RT-PCR的结果发现CHEK1，RPA1，RPA2和RPA3的转录在XPF细胞内较明显地增加，说明了Fe3O4纳米颗粒通过改变XPF细胞内这些基因的转录，从而抑制其分裂和生长.

Fe3O4 Nanoparticles Are Cytotoxic to XPF Mutated Human Cells

With the increasing application of Fe3O4 nanoparticles, it is necessary to investigate their safety to human cells. It has been revealed in this study that the colony-forming ability of HeLa cells is not influenced by Fe3O4 nanoparticles, indicating that Fe3O4 nanoparticles do not exhibit cytotoxicity to those cells. However, Fe3O4 nanoparticles significantly inhibit the colony-forming ability of XPF cells, which contain mutated XPF gene and possess a deficient nucleotide excision repair (NER) pathway, with a concentration dependent pattern. Our data illustrate that Fe3O4 nanoparticles are cytotoxic to XPF cells. HeLa cells and XPF cells were incubated with Fe3O4 nanoparticles containing cell culture media. The mRNA level of several genes, which were involved in cell cycle regulation or the cellular response to stress, has been detected by RT-PCR assay. Only significant elevated mRNA level of CHEK1, RPA1, RPA2 and RPA3 has been observed in XPF cells. The results imply that Fe3O4 nanoparticles are not suitable for patients with any mutated NER gene.