| 日本柑橘黄龙病病原体电镜观察及PCR检测 |
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| 引用本文： | 王运生,戴良英,荒井啓.日本柑橘黄龙病病原体电镜观察及PCR检测[J].湖南农业大学学报(自然科学版),2004,30(5):446-449. |
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| 作者姓名： | 王运生 戴良英 荒井啓 |
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| 作者单位： | 1. 湖南农业大学生物安全科技学院,湖南,长沙,410128
2. 日本国鹿儿岛大学农学部,日本,鹿儿岛,890-0054 |
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| 基金项目： | 湖南省科技厅资助项目(01NKY1002) |
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| 摘 要： | 对日本柑橘黄龙病病原体的形态结构进行了电镜观察，结果发现柑橘黄龙病病菌日本株系绝大多数是球形和短椭圆形，极少观察到棒状形态，直径为100～500nm，外被一层胶状物质包围，厚约10～50nm，这与其他地区发现的柑橘黄龙病病原体形态不同．PCR检测表明，以硅藻土法提取的DNA为模板，利用Ready-To-Go^TM PCR bead法可以得到理想结果。
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| 关 键 词： | 柑橘黄龙病 韧皮部杆菌 透射电镜 PCR检测 |
| 文章编号： | 1007-1032(2004)05-0446-04 |
| 修稿时间： | 2003年4月22日 |
Morphology of Japanese Citrus Huanglongbing Pathogen in Citrus Plant and It's Detection by PCR |
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| WANG Yun-sheng,DAI Liang-ying,Kei Arai.Morphology of Japanese Citrus Huanglongbing Pathogen in Citrus Plant and It''''s Detection by PCR[J].Journal of Hunan Agricultural University,2004,30(5):446-449. |
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| Authors: | WANG Yun-sheng DAI Liang-ying Kei Arai |
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| Institution: | WANG Yun-sheng1,DAI Liang-ying1,Kei Arai 2 |
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| Abstract: | The ultra-structure of the pathogen of Citrus Huanglongbing was observed on TEM. The results show that the morphology of the pathogen in Japan is spherical and oval in most time,very few in filamentous form. The organism is 100~500 nm in diameter and with an 10~50 nm envelope.This suggests that the Japanese strain a novel species.A rapid and accurate detection method based on PCR was also developed. |
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| Keywords: | Citrus Huanglongbing Candidatus liberibacter TEM PCR detection |
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