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| 细菌几丁质酶基因转化番茄的研究 | |
| 引用本文： | 任春梅,高必达,张学文,何迎春,苏建明.细菌几丁质酶基因转化番茄的研究[J].湖南农业大学学报(自然科学版),2002,28(4):298-301. |
| 作者姓名： | 任春梅 高必达 张学文 何迎春 苏建明 |
| 作者单位： | 1. 湖南农业大学理学院,湖南,长沙,410128 2. 湖南农业大学植物科学技术学院,湖南,长沙,410128 3. 湖南农业大学动物科学技术学院,湖南,长沙,410128 |
| 基金项目： | 湖南省教育厅重点资助项目 (97A 11) |
| 摘 要： | 为了获得抗真菌病的番茄材料，采用农杆菌介导法将细菌几丁质酶基因转化番茄子叶，获得了抗卡那霉素的转化植株，PCR检测初步证明细菌几丁质酶基因已整合到番茄的基因组。同时对番茄耐Kan水平、转化受体的预培养以及共培后洗涤抑菌进行了研究。结果表明：卡那霉素质量浓度为50mg/L时就能完全抑制番茄子叶再生；共培后直接用抑菌剂洗涤的抑菌效果较好；番茄子叶预培养1d的转化率最高。 |
| 关 键 词： | 细菌 几丁质酶 基因转化 番茄 农杆菌介导法 |
| 文章编号： | 1007-1032(2002)04-0298-04 |
| 修稿时间： | 2002年4月5日 |
Study on Tomato's Transformation with a Chitinase ChiA Gene from Serratia marcescens |
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| REN Chun mei ,GAO Bi da ,ZHANG Xue wen ,HE Ying chun ,SU Jian ming.Study on Tomato''s Transformation with a Chitinase ChiA Gene from Serratia marcescens[J].Journal of Hunan Agricultural University,2002,28(4):298-301. | |
| Authors: | REN Chun mei GAO Bi da ZHANG Xue wen HE Ying chun SU Jian ming |
| Institution: | REN Chun mei 1,GAO Bi da 2,ZHANG Xue wen 1,HE Ying chun 2,SU Jian ming 3 |
| Abstract: | For getting tomato that resists fungi diseases, a chitinase chiA gene from Serratia marcescens was transformed into tomato cotyledons by using Agrobacterium tumefaciens mediated method and transgenic plants with kanamycin resistance were obtained. The PCR examination has confirmed that the chitinase chiA gene is integrated into tomato genome. Furthermore, the anti Kan level of tomato, pre culture of cotyledons and washing cotyledons co cultured were studied. The results have shown that kanamycin at 50 mg/L can restrain tomato cotyledons regenerating completely, washing cotyledons co cultured with antibiotic controls growth of Agrobacteria tumefaciens more effectively and the highest transformation rate is got by using cotyledons pre cultured 1 day. |
| Keywords: | tomato bacterial chitinase gene Agrobacterium tumefaciens mediated transformation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |