| 基于ITS序列的菟丝子PCR鉴定 |
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| 引用本文： | 高必达,程毅,朱水芳,喻锦秀.基于ITS序列的菟丝子PCR鉴定[J].湖南农业大学学报(自然科学版),2006,32(4):368-370. |
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| 作者姓名： | 高必达 程毅 朱水芳 喻锦秀 |
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| 作者单位： | 1. 湖南农业大学,生物安全科学技术学院,湖南,长沙,410128
2. 中国检验检疫科学研究院,动植物检疫研究所,北京,100029 |
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| 基金项目： | 国家质量监督检验检疫总局科研项目 |
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| 摘 要： | 提取了7个药用菟丝子种样品的DNA，采用ITS序列扩增通用引物对其进行PCR扩增，并对PCR产物进行克隆和测序．根据所测得菟丝子ITS全长序列与其常见易混品所在属的代表植物ITS序列比对，设计了一对特异性引物并进行了PCR检测．结果表明，此对引物能将药用菟丝子种子与4种易混品区分开来，可以用于药用菟丝子的真伪鉴定．
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| 关 键 词： | 菟丝子 ITS序列 分子鉴定 |
| 文章编号： | 1007-1032(2006)04-0368-03 |
| 修稿时间： | 2006年4月5日 |
PCR Identification of Cuscuta Based on ITS Sequence |
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| GAO Bi-da,CHENG Yi,ZHU Shui-fang,YU Jing-xiu.PCR Identification of Cuscuta Based on ITS Sequence[J].Journal of Hunan Agricultural University,2006,32(4):368-370. |
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| Authors: | GAO Bi-da CHENG Yi ZHU Shui-fang YU Jing-xiu |
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| Abstract: | DNAs of 9 sets of samples from 7 officinal Cuscuta spp. were extracted.The ITS sequences by comparing th obtained sequences with the ITS sequences of 4 fake plant spieces in other family, the specific PCR primers wer designed and in further PCR detection proved effective in distinguishing Cuscuta sp.from the others. Finally it concluded that this method can be in practice used to identify officinal Cuscuta. |
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| Keywords: | Cuscuta ITS sequence PCR identification |
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