HP-20大孔吸附树脂分离纯化儿茶素EGCg的效果

以EGCg(表没食子儿茶素没食子酸酯)含量为31.52%的儿茶素粗提物为原料,比较D202、XDA-1、D201、D900、LSA-7、HP-20等6种大孔吸附树脂对EGCg的静态吸附与解吸的效果,并应用最佳大孔吸附树脂HP-20对EGCg分别进行动态等梯度洗脱和梯度洗脱试验,优化HP-20大孔吸附树脂分离纯化EGCg的技术参数.结果表明,应用体积为125mL的柱进行等梯度洗脱时,1.8g上样量、2BV/h流速、40%乙醇体积分数、2BV洗脱体积,EGCg的纯度和得率分别达55.92%和95.22%;以同样的柱进行梯度洗脱时,1.8g上样量、30%乙醇洗脱、2BV/h流速、2BV洗脱体积、收集1～2BV的体积段,EGCg的纯度和得率分别达68.32%和72.08%.

Separation and purification of EGCg in catechins by HP-20 macroporous resin

The rude catechin extracts of EGCg(Epi-gallate-catechin-gallate) with 31.52% as raw material,the HP-20 macroporous resin was screened from the six kinds of macroporous resin of D202,XDA-1,D201, D900,LSA-7,HP-20 by comparing their effects of static adsorption and de-sorption on EGCg.The HP-20 macroporous resin was then applied for gradient elution and in-gradient elution experiment,and the technological parameters were optimized.The result showed that 1.8 g injection solution,2 BV/h flowing speed,40% ethanol,2 BV elution volume were the best parameters for gradient elution,and the purity and yield rate of EGCg reached 55.92% and 95.22% separately.While the parameters of 1.8 g injection solution,2 BV/h flowing speed,30% ethanol,2 BV elution volume and 1-2 BV collection volume were the best parameters for in-gradient elution,and the purity and the yield rate of EGcg reached 68.32% and 72.08% in sequence,which showed good application value in industry.