博爱八月黄柿组织培养影响因素研究

以博爱八月黄柿休眠芽、萌动芽和梢尖为外植体,研究了取材时期、消毒时间、消毒剂和植物生长调节剂对其组织培养的影响.结果表明,休眠芽、萌动芽和梢尖的萌芽率最高分别为70%、50%、30%,休眠芽(休眠期外植体)的萌芽率最高,且增殖能力强,12月6日前后为最佳取材时期,以休眠芽、萌动芽为外植体可建立初代培养,而梢尖不易建立初代培养.0.1%HgCl2对生长前期外植体消毒5min的效果最好,其污染率、褐化率较低,成活率最高;0.1%HgCl2对萌动期外植体消毒10min的效果较好,其污染率虽高,但褐变率最低,成活率也最高;比较消毒剂NaClO和HgCl2,0.1%HgCl2处理休眠期外植体15min的效果最好,成活率高达70.2%.博爱八月黄柿组织培养的适宜培养基为1/2MS+1mg/LZT+0.1mg/LIAA+4.0mg/L6-BA.

Effect factors on tissue culture of Boaibayuehuang Diospyros kaki

Using Boaibayuehuang Diospyros kaki dormant buds, sprout buds and shoot tips as explants, effect of sampling time, disinfection time, disinfectants and plant growth regulators on culture of their organizations. The results showed that highest germination ratio of dormant buds, sprout buds and shoot tip reached 70%, 50%, 30% respectively, that of dormant bud (dormant period explants) is the highest, and its proliferation ability is stronger; best sampling period is around December 6, using dormant buds, sprout buds as explants could establish the beginning of culture, while the beginning of shoot tip culture is not easy to be established. Using 0.1%HgCl2 to disinfect 5 min will be best to explant at its earlier growing period, its pollution rate, the browning rate is relatively lower, while its survival rate is the highest; it would be better to use 0.1% HgCl2 to disinfect explants for 10 min, although its pollution rate is higher, but its browning rate is the lowest, the survival rate is the highest; Comparison of disinfectants (NaClO and HgCl2) indicated the optimal level of disinfectant to explant in the dormant period is 0.1%HgCl2, the disinfection time is 15min,the survival rate is higher, reaching 70.2%. The most suitable culture medium for Boaibayuehuang Diospyros kaki is 1/2 MS+1 mg/L ZT +0.1 mg/L IAA +4.0 mg/L 6-BA.