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| 人α-干扰素基因在转基因草鱼中的表达 | |
| 引用本文： | 陈立祥,肖调义,汪冬庚,苏建明,陈开健,章怀云,张学文,符少辉.人α-干扰素基因在转基因草鱼中的表达[J].湖南农业大学学报(自然科学版),2001,27(3):177-178. |
| 作者姓名： | 陈立祥 肖调义 汪冬庚 苏建明 陈开健 章怀云 张学文 符少辉 |
| 作者单位： | 1. 湖南农业大学动物科学技术学院, 2. 湖南农业大学理学院, 3. 湖南农业大学科学技术处, |
| 基金项目： | 国家“8 6 3”计划资助项目 (10 1- 0 1- 0 5 ) |
| 摘 要： | 为提高草鱼对出血病的抗性，采用显微注射法，将克隆在鲤β－肌动蛋白基因启动子下游的人α－干扰素基因转移到草鱼受精卵中，获得了大量转基因个体。抽取转基因鱼血浆，以酶联免疫吸附法检测了转基因草鱼中人α－干扰素基因的表达情况。从672尾转基因草鱼中检测出158尾有α－干扰素的表达，阳性率为23．51％，其中表达水平最高个体血浆中α－干扰素质量浓度为1450pg/mL。基因表达量随季节和个体发育时期有所变化。 |
| 关 键 词： | 草鱼 人α－干扰素 酶联免疫检测 基因转化 基因表达 出血病 抗生 |
| 文章编号： | 1007-1032(2001)03-0177-02 |
| 修稿时间： | 2001年4月16日 |
Expression of Hu-IFN-α Gene in Transgenic Grass Carp |
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| CHEN Li-xiang,XIAO Tiao-yi,WANG Dong-geng,SU Jian-ming,CHEN Kai-jian,ZHANG Huai-yun,ZHANG Xue-wen,FU Shao-hui.Expression of Hu-IFN-α Gene in Transgenic Grass Carp[J].Journal of Hunan Agricultural University,2001,27(3):177-178. | |
| Authors: | CHEN Li-xiang XIAO Tiao-yi WANG Dong-geng SU Jian-ming CHEN Kai-jian ZHANG Huai-yun ZHANG Xue-wen FU Shao-hui |
| Abstract: | To raise carp's resistance to hemorrhage,a large amount of transgenic grass carp was obtained by transfering hu-IFN-α gene which was cloned under the control of the β-actin gene promoter of grass carp to the cytula of carp in the way of microinjection. ELISA detection of the gene expression of the transgenic grass carps was carried out by using the serum as samples.158 individuals were tested to be positive with a detectable hu-IFN-α expression level in 672 transgenic fish. The detectable ratio is 23.51% and the highest is 1 450 pg/mL in serum. The expression level of the transformed gene changes in different seasons and development stages. |
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