| 茶尺蠖核型多角体病毒湖北分离株的分子鉴定 |
| |
| 引用本文： | 毛迎新,刘明炎,龚自明,张忠信,谭荣荣.茶尺蠖核型多角体病毒湖北分离株的分子鉴定[J].湖南农业大学学报(自然科学版),2010,36(6):685-689. |
| |
| 作者姓名： | 毛迎新 刘明炎 龚自明 张忠信 谭荣荣 |
| |
| 作者单位： | 湖北省农科院果茶所,湖北省农科院果茶所,湖北省农科院果茶所,中国科学院武汉病毒研究所,湖北省农科院果茶所 |
| |
| 基金项目： | 湖北省"十一·五"科技攻关项目，湖北省农业科技创新中心项目 |
| |
| 摘 要： | 运用PCR技术扩增茶尺蠖核型多角体病毒安徽分离株(EcobNPV-AH)和湖北分离株(EcobNPV-HB)的同源重复序列2(hr2),并对湖北分离株的hr2进行克隆和序列测定.结果表明,茶尺蠖核型多角体病毒安徽分离株hr2扩增产物为2条DNA片段,大小约1300bp和1800bp,湖北分离株的hr2扩增产物为1条DNA片段,大小约1800bp,由基因型组成判断,EcobNPV-HB是新的地区分离株,其hr2全长1761个核苷酸,GC含量21.7%,编码540个氨基酸,具有重复性回文序列,较GenBank中登录的EcobNPV-AH的hr2多362个核苷酸.
|
| 关 键 词： | 茶尺蠖核型多角体病毒 湖北分离株 基因型 同源重复序列2 |
| 收稿时间： | 2010/5/27 0:00:00 |
| 修稿时间： | 2010/11/9 0:00:00 |
Molecular identification of Ectropis obliqua nucleopolyhedrovirus isolated from Hubei strains |
| |
| maoyingxin,liumingyan,gongziming,zhangzhongxin and tanrongrong.Molecular identification of Ectropis obliqua nucleopolyhedrovirus isolated from Hubei strains[J].Journal of Hunan Agricultural University,2010,36(6):685-689. |
| |
| Authors: | maoyingxin liumingyan gongziming zhangzhongxin and tanrongrong |
| |
| Institution: | Institute of Fruit & Tea, Hubei Academy of Agricultural Sciences,Institute of Fruit & Tea, Hubei Academy of Agricultural Sciences,Institute of Fruit & Tea, Hubei Academy of Agricultural Sciences,Wuhan Institute of Virology, Chinese Academy of Sciences,Institute of Fruit & Tea, Hubei Academy of Agricultural Sciences |
| |
| Abstract: | Homologous repeat region 2 (hr2) of Ectropis obliqua nucleopolyhedrovirus (EcobNPV) isolated from Hubei and Anhui strains were amplified by PCR. The results showed that only one 1.8 kb DNA fragment was amplified from EcobNPV-HB strains and two DNA fragments was amplified from EcobNPV-AH strains, which suggested that EcobNPV-HB strains was an independent strains different from EcobNPV-AH strains. hr2 of Ectropis obliqua nucleopolyhedrovirus isolated from Hubei strains was cloned into pMD18-T vector, sequenced and analyzed. The hr2 of EcobNPV-HB strains was composed of 1 761 nucleotides and coded 540 amino acids with a G+C content of 21.7%, comprised of perfect palindromes. Extra 362 nucleotides were observed compared with hr2 of EcobNPV-AH strains in GenBank. |
| |
| Keywords: | Ectropis obliqua nucleopolyhedrovirus Hubei strains genotype homologous repeat region 2 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《湖南农业大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《湖南农业大学学报(自然科学版)》下载免费的PDF全文 |
|
