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| 临湘市具尾尖突松材线虫病病原的鉴定及病源分析 | |
| 引用本文： | 夏永刚,王明旭,张玉荣,肖启明,徐志刚.临湘市具尾尖突松材线虫病病原的鉴定及病源分析[J].湖南农业大学学报(自然科学版),2009,35(3). |
| 作者姓名： | 夏永刚 王明旭 张玉荣 肖启明 徐志刚 |
| 作者单位： | 1. 湖南省林业科学院,湖南,长沙,410004 2. 湖南省森林植物园,湖南,长沙,410116 3. 湖南农业大学,生物安全科学技术学院,湖南,长沙,410128 |
| 摘 要： | 运用形态鉴定和实时荧光定量PCR技术,对湖南省临湘市首次发现的具尾尖突的松材线虫病病原进行了确认,并对其rDNA区的ITS区特异性扩增、测序,病源分析发现其与松材线虫(JN0803)在ITS区内同源性为99.8%. |
| 关 键 词： | 松材线虫 病原鉴定 病源分析 临湘市 |
Indentification and origin analysis of Bursaphelenchus xylophilus with terminal mucroin Linxiang city |
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| XIA Yong-gang,WANG Ming-xu,ZHANG Yu-rong,XIAO Qi-ming,XU Zhi-gang.Indentification and origin analysis of Bursaphelenchus xylophilus with terminal mucroin Linxiang city[J].Journal of Hunan Agricultural University,2009,35(3). | |
| Authors: | XIA Yong-gang WANG Ming-xu ZHANG Yu-rong XIAO Qi-ming XU Zhi-gang |
| Institution: | 1.Hunan Forestry Academy;Changsha 410004;China;2.Hunan Forest Botanical Garden;Changsha 410116;3.College of Bio-Safety Science and Technology;HNAU;Changsha 410128;China |
| Abstract: | The first discovery of Bursaphelenchus xylophilus with terminal mucro was confirmed in Linxiang city through morphological identification and real-time fluorescence quantitative PCR technology,and its rDNA region ITS area was specifically amplified and sequenced,pathogenic analysis indicated that its homology was 99.8% in ITS area compared with Bursaphelenchus xylophilus(JN0803). |
| Keywords: | Bursaphelenchus xylophilus pathogenic indentification origin analysis Linxiang city |
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