海南地方猪POU1F1基因多态性与体质量的相关性

采用 PCR–SSCP、PCR–RFLP 技术,对海南五指山猪封闭群及其近交系、海南黑猪2个品系(临高猪、屯昌猪)的 POU1F1基因外显子4、外显子5、外显子6、内含子3的多态性进行研究.结果表明:猪 POU1F1基因外显子5、外显子6不存在多态性,内含子3、外显子4存在2个等位基因和3种基因型;在外显子4基因座上,五指山猪、临高猪、屯昌猪呈 Hardy–Weinberg 平衡状态,五指山猪近交系呈不平衡状态,4个猪种群的遗传多态性均处于中度多态(0.250.5);利用 SPSS 软件分析五指山猪 POUIF1基因内含子3和外显子4基因的多态性与其3个生长阶段体质量的相关性,发现引物 P1与 P4不同基因型个体体质量的差异均不显著

Association of POU1F1 gene polymorphism with growth traitsin Hainan native pig breeds

The PCR-SSCP and PCR-RFLP in exon 4,exon 5,exon 6 and intron 3 of POU1F1 gene and their correlations with growth traits were investigated in Hainan WZS pig in a closed breeding colony and their inbred line and in Hainan black pigs(Lingao pig and Tunchang pig).The results showed there were two alleles and three genotypes in exon 4 and intron 3 of POU1F1 gene and there were no polymorphism in exon 5 and exon 6.They were Hardy-Weinberg equilibrium state in exon 4 of POU1F1 gene in WZS pig,Lingao pig and Tunchang pig,while WZS pig inbred line showed unequilibrium state,and the four breeds all had intermediate genetic polymorphism(0.250.50).The least square analysis showed that the different genotype of exon 4 and intron 3 of POUlF1 gene did not affect body weight of 2,8 and 12-month-old pigs(P>0.05).