辣椒多态性ESTSSR标记开发及连锁图谱构建

采用SSR检索程序,从辣椒221 037条unigenes中筛选到17 319个SSR位点,设计了10 468对引物,并对其进行E-PCR多态性检测。结果表明,1 538对引物具有多态性,其中554条辣椒EST序列能匹配到番茄基因组上,构成了12条辣椒EST-SSR连锁群,平均每条连锁群含45.75个EST-SSR。对匹配到番茄基因组上的EST-SSR进行GO(gene ontology)分类,结果有481条EST序列被分类,其中以初生代谢、细胞代谢、生物合成过程为主,分别占34.13%、28.35%、25.82%。在KEGG map中,91条EST序列共涉及到76条代谢途径(KEGG),约67条序列参与新陈代谢途径,32条序列参与次生代谢产物合成途径。

Development of pepper EST-SSRs and an SSR-based linkage map

In this study,17 319 SSRs were identified from 221 037 ESTs of pepper using SSR finding soft.A total of 10 468 primer pairs were successfully designed and 1 538 primer pairs exhibited polymorphism by E-PCR.Among the polymorphic primer-containing ESTs,554 ESTs containing SSR motifs were matched onto the tomato genome,forming 12 linkage groups with an average of 45.75 makers per group.Analysis of GO(gene ontology) showed that 481 ESTs were classified mainly to be involved in primary metabolic process,cellular metabolic process and biosynthetic process.In the KEGG map,91 ESTs were involved in 76 pathways(KEGG),67 ESTs took part in metabolic pathways and 32 participated in biosynthesis of second metabolites.