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| 苎麻叶片高效再生体系的建立 | |
| 引用本文： | 曹雅琴,刘峰,陈建荣,郭清泉.苎麻叶片高效再生体系的建立[J].湖南农业大学学报(自然科学版),2009,35(2). |
| 作者姓名： | 曹雅琴 刘峰 陈建荣 郭清泉 |
| 作者单位： | 1. 湖南农业大学,苎麻研究所,湖南,长沙410128 2. 湖南省农业科学院,蔬菜研究所,湖南,长沙410125 3. 长沙学院,生物工程与环境科学系,湖南,长沙,410003 |
| 摘 要： | 以湘苎2号、湘苎3号、新宁青麻、四川洪县园麻的叶片为材料,构建4个品种高效、稳定的不定芽再生体系. 湘苎2号、湘苎3号、新宁青麻、四川洪县园麻最适诱导愈伤组织及分化不定芽的培养基分别为MS+0.7 mg/L TDZ+0.02 mg/L 2,4-D,MS+0.3 mg/L TDZ+0.01 mg/L 2,4-D,MS+0.3 mg/L TDZ+0.05 mg/L 2,4-D,MS+ 0.9 mg/L TDZ +0.05 mg/L 2,4-D;不定芽分化率分别为83.3%,81.7%,90.0%,86.7%;4个品种再分化时出现丛生芽,新宁青麻愈伤组织的平均再生不定芽数达2.62个,其他3个品种也都有数量不等的丛生芽产生,不定芽生根培养基为MS+ 0.05 mg/L NAA;叶块经过添加6%蔗糖培养基培养2周,再转入含3%蔗糖的培养基中培养,其中经6%蔗糖培养能显著提高湘苎2号、新宁青麻、四川洪县园麻不定芽分化率、平均再生不定芽数. |
| 关 键 词： | 苎麻 愈伤组织 不定芽 再生体系 |
High-frequency plant regeneration system from leaf explants of ramie |
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| CAO Ya-qin,LIU Feng,CHEN Jian-rong,GUO Qing-quan.High-frequency plant regeneration system from leaf explants of ramie[J].Journal of Hunan Agricultural University,2009,35(2). | |
| Authors: | CAO Ya-qin LIU Feng CHEN Jian-rong GUO Qing-quan |
| Institution: | 1.Ramie Research Institute;HNAU;Changsha 410128;China;2.Institute of Vegetable;Hunan Agricultural Academy of Science;Changsha 410125;3.Department of Biology and Environmental Science;Changsha College;Changsha 410003;China |
| Abstract: | An efficient plant regeneration systerm via adventitious shoot formation from leaf explants of ramie cultivar: Xiangzhu 2,Xiangzhu 3,Xinningqingma and Sichuanhongxianyuanma were established.The best medium for shoot regeneration of the four ramie cultivar are:Xiangzhu 2 is cultivated on MS medium supplenmented with 0.7 mg/L TDZ and 0.02 mg/L 2,4-D,Xiangzhu 3 is cultivated on MS medium supplenmented with 0.3 mg/L TDZ and 0.01 mg/L 2,4-D,Xinningqingma is cultivated on MS medium supplenmented with 0.3 mg/L TDZ... |
| Keywords: | ramie callus adventitious shoot regeneration system |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |