| 雄性不育基因barnase对西瓜的遗传转化 |
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| 引用本文： | 陈银华,张广平.雄性不育基因barnase对西瓜的遗传转化[J].湖南农业大学学报(自然科学版),2006,32(2):128-130. |
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| 作者姓名： | 陈银华 张广平 |
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| 作者单位： | 1. 海南大学,生命科学与农学院,海南,海口,570228;海南省耐盐作物生物技术重点实验窒,海南,海口,570228
2. 中南大学,生物科学与技术学院,湖南,长沙,410125 |
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| 基金项目： | 国家自然科学基金项目(30270914) |
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| 摘 要： | 将带有雄性不育基因、除草剂基因及卡那霉素抗性基因的质粒pBI-TBSbar通过冻融法导入根癌农杆菌菌株LBA4404中,采用农杆菌介导法转化西瓜品种(京欣一号,亲本自交系C-1),获得具有卡那霉素抗性植株8株,PCR,Southern blot检测表明,外源片段已经整合到西瓜基因组中.
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| 关 键 词： | 西瓜 雄性不育基因barnase 除草剂基因bar 遗传转化 |
| 文章编号： | 1007-1032(2006)02-0128-03 |
| 修稿时间： | 2005年10月11 |
Genetic Transformation of Watermelon with the Male Sterility Gene-Barnase |
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| CHEN Yin-hua,ZHANG Guang-ping.Genetic Transformation of Watermelon with the Male Sterility Gene-Barnase[J].Journal of Hunan Agricultural University,2006,32(2):128-130. |
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| Authors: | CHEN Yin-hua ZHANG Guang-ping |
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| Abstract: | The plasmid pBI-TBSbar carried the barnase,bar and NPTII were successfully transferred into two watermelon cultivars by Agrobacterium-mediated tansformation.Regenerated plantlets were obtained from cotyledons after preculture,shoot inducing culture and rooting culture.Transgenic plants were confirmed by the kanamycin-resistance experiment,PCR analysis and Southern blot. |
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| Keywords: | watermelon male sterility gene(barnase) herbicide resistance gene transformation |
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