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烟叶面细菌分离筛选及其对烟草赤星病的拮抗作用
引用本文:罗坤,罗宽,朱小湘,何昆,黎起秦,黄世文.烟叶面细菌分离筛选及其对烟草赤星病的拮抗作用[J].湖南农业大学学报(自然科学版),2006,32(3):245-247.
作者姓名:罗坤  罗宽  朱小湘  何昆  黎起秦  黄世文
作者单位:1. 广西大学,农学院,广西,南宁,530000
2. 湖南农业大学,生物安全科学与技术学院,湖南,长沙,410128
3. 中国水稻研究所,浙江,杭州,310006
基金项目:湖南省烟草专卖局科研项目
摘    要:为了从烟叶面获得防治烟草赤星病的拮抗菌,利用稀释分离方法,从湖南、云南烟区烟株下部叶片分离得到烟草叶面细菌676株,通过对峙培养试验、孢子萌发试验,结果16株菌株对烟草赤星病有较强拮抗作用;室内盆栽生防测定试验表明,16株菌株不同程度地减轻了烟草赤星病的发生,其中18,19,29,49,71号防治效果好,其防效分别为77.4%,77.4%,92.5%,92.5%和90.3%.

关 键 词:烟草叶面细菌  烟草赤星病  生物防治
文章编号:1007-1032(2006)03-0245-03
修稿时间:2006年2月27日

Screening and Choosing of Antagonistic Bacteria from Phylloshere and Initially Determining Its Resistance to Alternaria alternata
LUO Kun,LUO Kuan,ZHU Xiao-xiang,HE Kun,LI Qi-qin,HUANG Shi-wen.Screening and Choosing of Antagonistic Bacteria from Phylloshere and Initially Determining Its Resistance to Alternaria alternata[J].Journal of Hunan Agricultural University,2006,32(3):245-247.
Authors:LUO Kun  LUO Kuan  ZHU Xiao-xiang  HE Kun  LI Qi-qin  HUANG Shi-wen
Abstract:In order to get antagonistic bacteria for controlling tobacco brown spot,from the smoke area of Hunan and Yunnan,676 stains of bacteria were isolated from the phyllospheres tobacco of lower part leaf,using dilution asunder method.And 16 of these possessed high level of antagonistic properties by the result of the test of confronting each other and controlling bourgeon spore.By strain 18,19,29,49,71 used as bio-control agent against tobacco brow spot in greenhouset,he results showed that the three strains had strong inhibitive effect.The inhibitive efficacy was respectively 77.4%,77.4%,92.5%,92.5% and 90.3%.
Keywords:bacteria isolated from the phyllosheres of tobacco  Alternaria alternata  biological control
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