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甘蓝型油菜FAD2基因cDNA片段的克隆和序列分析
引用本文:熊兴华,官春云,李栒,王学军,周小云,李家洋.甘蓝型油菜FAD2基因cDNA片段的克隆和序列分析[J].湖南农业大学学报(自然科学版),2002,28(2):97-99.
作者姓名:熊兴华  官春云  李栒  王学军  周小云  李家洋
作者单位:1. 作物基因工程湖南省重点实验室,湖南,长沙,410128
2. 中国科学院遗传研究所,北京,100101
基金项目:湖南省科技厅资助项目 (97JKY10 0 5 )
摘    要:为了提高油菜的油酸含量,继而培育高油酸油菜品种,从甘蓝型油菜成熟叶片中分离总RNA,经反转录合成cDAN第一条链,以此为模板进行多聚酶链式反应,产物为一长654bp的DNA片段,经克隆和序列测定表明,其核苷酸序列与GenBank中甘蓝型油菜FAD2基因在比较区的同源性达100%。

关 键 词:甘蓝型油菜  FAD2基因  cDAN片段  基因克隆  序列分析  PCR  品质改良  油菜脱氢酶
文章编号:1007-1032(2002)02-0097-03
修稿时间:2001年11月14

Cloning and Sequencing of a cDNA Sequence Encoding FAD2 from Brassica napus
XIONG Xing hua ,GUAN Chun yun ,LI Xun ,WANG Xue jun ,ZHUO Xiao yun ,LI Jia yang.Cloning and Sequencing of a cDNA Sequence Encoding FAD2 from Brassica napus[J].Journal of Hunan Agricultural University,2002,28(2):97-99.
Authors:XIONG Xing hua  GUAN Chun yun  LI Xun  WANG Xue jun  ZHUO Xiao yun  LI Jia yang
Institution:XIONG Xing hua 1,GUAN Chun yun 1,LI Xun 1,WANG Xue jun 1,ZHUO Xiao yun 1,LI Jia yang 2
Abstract:To increase the oleate contents in rape and breed a rape variety of high oleate contents,the first strand of cDNA from the total RNA extracted from leaf of rapeseed( Brassica napus cv XY15) was synthsized and was used as a template for PCR reaction with oligonucleotide as a primer synthesized according to the FAD2 gene from oilseed rape. The PCR products were cloned into pGEM T Easy and sequenced. The results show that the cloned fragment contains 654 nucleotides,and shares a homology of 100% in comparison part with the reported data.
Keywords:Brassica napus  PCR  oleate desaturase cDNA
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