壳聚糖对草鱼非特异性免疫功能的影响

选择平均体重(70.61±1.29)g的健康草鱼450尾,随机分成5个处理组,即基础饲料组、抗生素组、0.25%、0.50%及1.00%壳聚糖组,每个处理设3个重复,每个重复放养30尾鱼,进行为期40d的饲养试验.结果表明,日粮中添加壳聚糖能显著增加草鱼的后肾体指数(P0.05),对草鱼的头肾体指数和脾体指数影响不显著,但与基础饲料组相比,各壳聚糖添加组草鱼的头肾体指数和脾体指数有增加趋势(P0.05).添加0.50%壳聚糖能显著增加草鱼血清总蛋白含量(P0.05),对草鱼血清和肝胰脏总SOD活力影响不显著(P0.05),但有增加趋势.即草鱼日粮中添加适量的壳聚糖能提高机体非特异性免疫能力,其适宜添加剂量为0.50%.

Effects of chitosan on nonspecific immunity in grass carps

To investigate effects of chitosan on nonspecific immunity in grass carps, five test groups were used, which consisted of control group, antibiotics group, 0.25%, 0.50% and 1.00% chitosan addition group. 450 grass carps with average body weight (70.61±1.29) g were randomly allocated to five groups with three replicates and with 30 grass carps in each replicate and were fed for 40 d. The results showed that, post kidney body index increased significantly by adding chitosan, while head kidney body index and spleen body index showed no significant difference (P>0.05) among groups. 0.5% chitosan significantly impacted on serum total protein in grass carp (P<0.05), however serum SOD and hepatopanereas SOD showed no significant difference (P>0.05) among groups. Above results suggested that, chitosan could significantly increase nonspecific immunity in grass carps and the appropriate chitosan addition was 0.50%.