| 南方水稻黑条矮缩病毒外壳蛋白p1O的原核表达和抗血清制备及应用 |
| |
| 引用本文： | 张蔚明,刘燕娟,廖晓兰.南方水稻黑条矮缩病毒外壳蛋白p1O的原核表达和抗血清制备及应用[J].湖南农业大学学报(自然科学版),2011,37(4):400-402. |
| |
| 作者姓名： | 张蔚明 刘燕娟 廖晓兰 |
| |
| 作者单位： | 湖南农业大学生物安全科学技术学院,湖南农业大学生安院,湖南农业大学生安院 |
| |
| 摘 要： | 南方水稻黑条矮缩病毒(SRBSDV) p10蛋白由其基因组片段S10编码,根据SRBSD海南分离物S10序列(EU523360)设计特异性引物扩增编码p10蛋白的片段,并亚克隆至原核表达载体pET-28a+,以大肠杆菌BL21 plysS为宿主菌进行高水平表达,利用纯化的p10蛋白免疫兔子,制备了p10蛋白的特异性抗血...
|
| 关 键 词： | 南方水稻黑条矮缩病毒 原核表达 抗血清 |
| 收稿时间： | 2010/11/29 0:00:00 |
| 修稿时间： | 2011/3/7 0:00:00 |
Prokaryotic expression of p10 protein of Southern rice black-streakeddwarf Fijivirus and preparation and application of its antiserum |
| |
| Institution: | HUNAN AGRICULTURAL UNIVERSITY,, |
| |
| Abstract: | Abstract: Protein p10 is encoded by the genome segment S10 of Southern rice black-streaked dwarf virus (SRBSDV).Based on the sequence of S10 (EU523360), a pair of S10 specific primers were designed and used to amplify the p10 protein-encoding gene. The expected product was subcloned into the expression vector pET28a+, which was transformed into Escherichia coli BL21plysS and expressed at high level. Antiserum specifically against p10 protein was prepared using the purified protein to immunize rabbit. ELISA tests showed that the antiserum against p10 protein could react strongly with fluids from diseased insects, indicating that the antiserum could be used to rapidly diagnose the SRBSDV infection in fields. |
| |
| Keywords: | Key words: Southern rice black-streaked dwarf virus (SRBSDV) prokaryotic expression antiserum |
|
| 点击此处可从《湖南农业大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《湖南农业大学学报(自然科学版)》下载免费的PDF全文 |
