以luxAB为报告基因的大豆根瘤菌的竞争结瘤研究

将来自pHN101的luxAB基因和来自pTR102的parCBA /DE基因,经过一系列中间载体整合到PLAFR3上构建成广宿主、稳定性质粒PHN158。通过三亲本杂交将pHN158导入费氏中华根瘤菌得到4株能在癸醛的激发下发出荧光的转移接合子WZRL、HWRL、HZRL和WWRL。将它们与3株大豆慢生根瘤菌WHB1、WHB2和WWB组配成12对组合,并以黑龙33和Williams为宿主以luxAB为报告基因进行竞争结瘤试验。结果表明,费氏中华根瘤菌与大豆慢生根瘤菌之间的平均竞争结瘤能力相当;同类型根瘤菌不同菌株之间的竞争结瘤能力存在着显著的差异;同时根瘤菌的竞争结瘤能力也明显受到宿主的影响。表明费氏中华根瘤菌与大豆慢生根瘤菌之间的竞争结瘤是一个菌株之间、菌植之间复杂的相互作用的过程。

Study on the Competitive Nodulation of Soybean Rhizobia by Using the Report Gene luxAB

A broad host range, stable plasmid, pHN158, was constructed by cloning of luxAB from pHNlOl and parCBA/DE from pTR102. It was then transferred into Sinorhizobium fredii strains and 4 transconjugants WZRL, HWRL, HZRL and WWRL showing fluorescence were obtained. Twelve pairs of strains from 4 transconjugant and 3 Bradyrhizobium japonicum strains were inoculated on the cultivars of Heilong 33 and Williams. The results of competitive nodulation tests showed that the average of nodule occupancies between fast and slow-growing strains was equal but there were significant differences between strains of the same type rhizobia. The influences of host plants on strain nodulation which shown the interaction between rhizobium srain and plant were also found.