侵染广州番木瓜的曲叶病毒DNA-A分子特征及生物学测定

 从中国广州番木瓜上检测获得双生病毒分离物GT，核苷酸序列测定表明，GT分离物 DNA-A全长2 769个核苷酸，编码6个ORFs，其中病毒链编码AV1（CP）和AV2两个ORFs，互补链编码AC1～AC4四个ORFs。DNA-A全序列、基因间隔区核苷酸序列及各ORF编码的氨基酸序列比较表明，GT与广东番木瓜曲叶病毒分离物GD2亲缘关系最近（96.7％）。生物学初步测定表明，该病毒可通过烟粉虱（Bemisia tabaci）传播到番木瓜、烟草和番茄植株上。

Molecular Characterization of Papaya leaf curl virus Infecting Carica papaya in Guangzhou and Its Biological Test

A geminivirus isolate GT infecting papaya was detected and obtained from Guangzhou,Guangdong Province.The complete nucleotide sequence of DNA-A of GT was determined to be 2 769 nucleotides in length,and had typical genomic organization of a begomovirus,encoding six ORFs with two ORFs AV1(CP) and AV2] in virion-sense DNA and four ORFs (AC1-AC4) in complementary-sense DNA.The comparison of complete and intergenic region nucleotide sequences and encoded amino acid sequences for ORFs with other begomoviruses indicated that GT had the highest sequence identity with isolate papaya leaf curl Guangdong virus isolate GD2.The biological test of isolate GT suggested that GT could infect tobacco,tomato and papaya by whiteflies (Bemisia tabaci) transmission.